Isolation and Inoculation Effect of Dark Septate Endophytes (DSE) From Ormosia hosiei
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2018年22
- Page:
- 83-87
- Research Field:
- Publishing date:
Info
- Title:
- Isolation and Inoculation Effect of Dark Septate Endophytes (DSE) From Ormosia hosiei
- Author(s):
- LIU Yan1; 2; WEI Xiaoli1
- (1.College of Forestry,Guizhou University，Guiyang,Guizhou 550025；2.Guizhou Institutes of Biology，Guiyang,Guizhou 550009)
- Keywords:
- Ormosia hosiei; dark septate endophyte; isolation; inoculation effect
- PACS:
- -
- DOI:
- 10.11937/bfyy.20181296
- Abstract:
- Seedlings of Ormosia hosiei was taken as test material,endophytic fungi was isolated from roots to investigate dark septate endophyte.The results showed that five strains of endophytic fungi were obstained using tissue isolation method and morphological characteristics.A pot culture experiment was showed that two strains colonized and formed dark septate hyphae and microsclerotia.These results confimed that two strains were dark septate endophytes fungi.Two strains were identified as Acrocalymma vagum and Leptosphaeria sp.AS71 based on rDNAITS sequence analysis.Coculture experiment showed that two strains had prominent effects on promoting plantlet height which were increased by 49.48% and 24.80%.Simultaneously,dry weight increased by 63.63% and 36.36% at significant level compared with the control.Dark septate endophyte had signficant promoting effects of growth.
Last Update: 2019-01-08