Culture Technology for Aseptic Seedling of Pumpkin
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2018年21
- Page:
- 22-29
- Research Field:
- Publishing date:
Info
- Title:
- Culture Technology for Aseptic Seedling of Pumpkin
- Author(s):
- WU Xixi; ZHANG Enhui; CHENG Yong′an; WANG Gaigai; CAO Lihong; YIN Panpan
- (College of Agriculture,Northwest A & F University,Yangling,Shaanxi 712000)
- Keywords:
- pumpkin（Cucurbita moschata）; seed; tissue culture; germination; aseptic seedling
- PACS:
- -
- DOI:
- 10.11937/bfyy.20181035
- Abstract:
- The ‘Miben’ pumpkin seeds were used as materials,the influence of seed skin treatment,the type of membrane treatment,pregermination,the agar and sucrose concentration of culture medium on germination and tissue culture seedling were studied,for which a serious of indices such as radicle rate,lateral roots rate,ratio of cotyledon turning green,cotyledon expansion rate and first main leaf rate were analyzed,and differences of leaf blade length,leaf blade width,leaf blade coarseness,internode length,plant height and growing point height were measured to obtain the optimum cultivation system of pumpkin sterile seedling.This best condition of aseptic tissue culture pumpkin seedling cultivation was researched,to provide support for pumpkin breeding.The results showed that,the better way for pumpkin seeds to get aseptic seedlings was to peel the seed shell and preserve seed membrane,the true leaf differentiation appeared the earliest when agar concentration was 5 g·L-1,but the plants gradually appeared vitrified;the comprehensive properties of the sterile seedlings were optimal when agar concentration was 6 g·L-1 or 8 g·L-1;plant germination as well as seedling early growth and development went the fastest when medium was not added with sugar,plant late vegetative growth was the most exuberant when added with 30 g·L-1 sugar.The influence factors of aseptic culture of pumpkin seedlings were discussed,among which pumpkin seeds were peeled with membrane intact and sown in MS medium with 6 g·L-1 agar and 30 g·L-1 sugar,to provide support for breeding pumpkin propagation and genetic transformation.
Last Update: 2018-12-19