Abstract

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Title:: Isolation of Wild Cordyceps militaris and Preliminary Study on Its Molecular Identification of Growing Grass

Author(s):: XIAO Jun1; XIA Shuang2; CHEN Xun1; ZHAO Ying1; (1.Edible Fungi Research Institute,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161;2.Liaoning GTYK Biology Technological Company Limited,Shenyang,Liaoning 110141)

Abstract:: In order to study the effect of the isolation of wild Cordyceps militaris fruiting body,and also to probe into the relationship between the wildtype strain and the matingtype gene,the collected wild C.militaris fruiting body were isolated and identified the mating type genes by PCR.The results showed that the 5 isolates which were separated and cultivated from the 11 isolates collected had distinct differences from each other in morphology.The wild isolates which had normal forms of fruiting body had 2 kinds of mating type genes or had the type of MATalpha mating gene only.The isolates which didn′t produce fruiting body contained two mating type genes simultaneously.Therefore,the separation of the fruiting bodies of wild C.militaris was the best way to help with the short of the current resources of C.militaris,and the lack of the mating type was not related to the growth of the fruiting body.