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Title:: Colonization Ability of Trichoderma harzianum Preparation and Effects on Microflora in Rhizosphere Soil of Watermelon

Author(s):: ZHANG Lirong1; CHEN Hang2; KANG Pingzhi1; DU Yuning1; ZHANG Zhike1; (1.Institute of Plant Protection,Ningxia Academy of Agriculture and Forestry Sciences,Ningxia Key Laboratory of Plant Disease and Pest Control,Yinchuan,Ningxia 750002;2.Institute of Agricultural Products Quality Standards and Technology,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002)

Keywords:: Trichoderma harzianum preparation; watermelon; colonization ability; microorganisms in rhizosphere soil

Abstract:: The soil of watermelon with continuous cropping 8 years was used as the test material,the colonization ability of 4 kinds of Trichoderma harzianum preparation in rhizosphere of watermelon was studied by using the traditional plate colony counting method,and its influence on rhizosphere soil microbial flora and Fusarium oxysporum was investigated by pot experiment.The results showed that the number of microbial populations in rhizosphere soil of watermelon was changed after the application of 4 Trichoderma harzianum preparation,and the effect of Ty3 preparation was the most prominent.The number of bacteria and actinomycetes in rhizosphere soil increased by 82.35% and 295.23%,respectively,compared with the control,and the number of fungi and Fusarium oxysporum decreased by 24.29% and 24.16%.The results of colonization test showed that the colonization of Trichoderma harzianum preparation in the rhizosphere soil of watermelon was the largest in the 14 days,and the Ty3 was increased by 14 077.08%,then basically remained stable,and was 156.7 times higher than control when applied for 56 days.The results showed that Trichoderma harzianum preparation could significantly increase the number of beneficial microorganisms in rhizosphere soil of watermelon,effectively inhibit the growth of the number of Fusarium oxysporum and reduce the rhizosphere micro-ecological effect of continuous cropping obstacle.