Abstract

Info

Title:: Induction of Seed Callus From Four Kinds of Kentucky Bluegrass Seeds With Different Hormones

Author(s):: CHEN Yang1; 2; SUN Huashan1; JIN Yifeng2; WANG Jiahe2; CHEN Yajun1; (1.College of Horticulture and Garden,Northeast Agricultural University,Harbin,Heilongjiang 150030;2.College of Life Science and Agriculture,Qiqihar University,Qiqihar,Heilongjiang 161006)

Abstract:: In this study,four Kentucky bluegrass varieties were used as materials for the screening of appropriate sterilization and callus induction methods with the mature seeds as explants,in order to supply reference for bluegrass use.The results showed that,the optimal sterilization method was to treat the seeds by 1% NaClO for 7 minutes followed by 4-5 times of washing by distilled water.Subsequently,the seeds were immersed with sterile water for 12 hours.Right before inoculation,the seeds were again washed with 75% ethanol for 40 seconds and then with sterile water for 4-5 times,followed by 10 minutes sterilization by 1% NaClO.The four Kentucky bluegrass varieties possessed different genotypes as well as significantly different frequencies of callus induction (P<0.05).The optimal culture medium was MS+1 mg·L-1 2,4-D+0.1 mg·L-1 6-BA for Kentucky bluegrass ‘Midnight II’,MS+6 mg·L-1 2,4-D for Kentucky bluegrass ‘Park’ and ‘Black Tiger’,and MS+1 mg·L-1 2,4-D for Kentucky bluegrass ‘Snow Wolf’,respectively.