Abstract

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Title:: Tissue Culture of Polygonatum cyrtonema Huad.With Leaf as Explants

Author(s):: CHEN Songshu1; 2; ZHANG Xue3; ZHAO Zhi1; 3; LIU Hongchang1; 3; WANG Hualei1; 3; LI Jinling1; 3; (1.Guizhou Key Laboratory of Propagation and Cultivation on Medicinal Plants,Guiyang,Guizhou 550025;2.Teaching Farm,Guizhou University,Guiyang,Guizhou 550025；3.Agricultural College,Guizhou University,Guiyang,Guizhou 550025)

Abstract:: To construct a rapid propagation system of the tissue culture of Polygonatum cyrtonema Huad.which regeneration from the leaves.The aim was to screen the optimum combination for the best medium of callus induction,callus proliferation,cluster bud induction and rooting induction of clustered shoots by adding different 6-BA,NAA,2,4-D and sucrose proportions in MS medium.The results showed that the best medium for callus induction was MS+6-BA 3.0 mg·L-1+NAA 4.0 mg·L-1+sucrose 20 g·L-1,and the callus induction rate was the highest,it reached 53.89%,and the growth was the best.The best medium for callus proliferation was MS+6-BA 2.0 mg·L-1+NAA 3.0 mg·L-1+sucrose 20 g·L-1,and the multiplication multiple was 7.87,and the growth was the best.The best medium for the induction culture of cluster buds was MS+6-BA 2.0 mg·L-1+NAA 4.0 mg·L-1,and the germination rate was the highest,it reached 86.11%,and the maximum height of 1.70 cm.The best medium of rooting induction for clustered shoots was MS+NAA 1.0 mg·L-1,and the rooting rate was the best,it reached 66.67%,and the best root number,length and diameter were 31.0,0.46 cm and 1.100 mm,respectively.A large amount of Polygonatum cyrtonema Huad.tissue culture seedlings could be obtained by the regeneration from the leaves,in a short time,which could solve the shortage of seedlings in planting production.