|Table of Contents|

Optimization of PAGE Silver-stained System Based on SSR Markers in the Chestnut

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年12
Page:
27-33
Research Field:
Publishing date:

Info

Title:
Optimization of PAGE Silver-stained System Based on SSR Markers in the Chestnut
Author(s):
NIE Xinghua1; ZHANG Qing1; 2; LIU Yang1; DAI Yuexing1; CAO Qingqin3; QIN Ling1
(1.College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206;2.Beijing Key Laboratory for Agricultural Application and New Technique,Beijing 102206;3.College of Biological Science and Engineering,Beijing University of Agriculture，Beijing 102206)
Keywords:
polyacrylamide gel; chestnut; orthogonal experiment; silver-stained effect
PACS:
-
DOI:
10.11937/bfyy.20180369
Abstract:
In order to screen out the high-efficiency and stable silver-stained system，in the study the DNA of chestnut leaves were used as the amplification template,and the four levels of AgNO3 usage amount,CH2O usage amount,Na2CO3 usage amount and sample volume were filtrated by L16(45) orthogonal experimental design,which were proved how these four main factors affect the polyacrylamide gel silver-stained effect.The improved method was analyzed in terms of silver-stained effect，reagent and silver-stained time.The results showed that under alkaline conditions，0.1% AgNO3+1 mL CH2O+0.2 g Na2CO3 (500 mL system)+1.5 μL samplevolume was the best.The selected system could effectively overcome the issues of low resolution，dark background，unclear read bands and high consumption of dangerous reagents.It provided support for batch experiments and fast reading.

References:

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Last Update: 2019-07-17