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Title:: Cloning,Characterization and Expression Analysis of ERF CmRAP2-12 in Chrysanthemum morifolium

Author(s):: LIAN Qinglong1; 2; TIAN Jing1; 2; LU Shaowei1; 2; MA Ning1; 2; BAO Shunshu1; 2; LI Shao1; 2; (1.Institute of Facility Agriculture,Chinese Academy of Agricultural Engineering,Beijing 100125；2.Key Laboratory of Farm Building in Structure and Construction,Ministry of Agriculture,Beijing 100125)

Keywords:: Chrysanthemum morifolium; ethylene response factor(ERF); waterlogging stress; hypoxic; expression

Abstract:: In this study,a full-length cDNA named CmRAP2-12 was cloned in Chrysanthemum morifolium ‘Jinba’ roots by RT-PCR and RACE,and the real time RT-PCR was used to analyze the expression pattern of the three genes.The results showed that cDNA sequence of CmRAP2-12 obtained in this study was 1 335 bp,CmRAP2-12 conserved N-terminal amino acids contained a Ⅶ ERFs subfamily characteristic amino acid sequence,and clustered to RAP2-12 subfamily. CmRAP2-12 was predicted subcellular localization in the nucleus.Real-time RT-PCR analysis showed that CmRAP2-12 was all expressed in root,stem and leaf in Chrysanthemum.CmRAP2-12 expression levels presented a first increased and then decreased trend,after waterlogging stress 0-72 hours.It was treated after 48 hours reached the highest value.The expression levels of ethylene response transcription factor 〖STBX〗RAP2-12 〖STBZ〗was increased in Chrysanthemum roots and affected by hypoxic response when suffering from waterlogging stress.This study would provide the theoretical basis for further research 〖STBX〗RAP2-12 〖STBZ〗to improve chrysanthemum flooding tolerance to hypoxia and molecular mechanisms regulating response mechanism.