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Title:: Optimization of Microwave Assisted Extraction of Artemisia annua L.Polysaccharides by Response Surface Methodology

Author(s):: WEI Zengyun1; ZHAO Feiyan2; ZHANG Hairong2; (1.College of Xinzhou Occupation Technology,Xinzhou,Shanxi 034000;2.Institute of Biochemical Analysis,Xinzhou Teachers′ College,Xinzhou,Shanxi 034000)

Keywords:: microwave-assisted extraction; response surface methodology; Artemisia annua L. polysaccharides

Abstract:: With wild Artemisia annua L.from Xinzhou as an experimental material,Box-Behnken center combination was used to optimize the technology of the microwave-assisted extraction of Artemisia annua L.polysaccharides by response surface methodology.Multiple regression analysis for 3 factors and 3 levels center combinations was performed with the Design-Expert 7.1.6 system software on the microwave power,extraction time and liquid-material ratio.The results showed that,the fitting regression equation was Y=0.44+2.125×10-3A-0.017B-0.021C+7.750×10-3AB+0.012AC+0.011BC-0.04A2+0.022B2-0.02C2.The correlation coefficient of the model,i.e.,R2=0.945 3,the model was extremely significant,it could be used to predict extraction test results of artemisia annua L. polysaccharide.The optimal parameters of microwave-assisted extraction of polysaccharides from Artemisia annua L.were microwave power of 450 W,extraction time of 71 seconds,liquid-material ratio of 60∶1 mL·g-1.Artemisia annua L.polysaccharide extraction rate was up 9.72% under the optimal condition.The experiment could provide a useful reference for industrial extraction of polysaccharide from Artemisia annua L..