Cloning and Expression Analysis of a Transcription Factor Gene ZjDREB1.2 From Zoysia
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2018年18
- Page:
- 75-85
- Research Field:
- Publishing date:
Info
- Title:
- Cloning and Expression Analysis of a Transcription Factor Gene ZjDREB1.2 From Zoysia
- Author(s):
- FENG Wanqian; MA Lipeng; JIANG Xiaoxiao; ZHANG Hong; LI Yue; WEI Shanjun
- (Department of Life and Environmental Science,Minzu University of China,Beijing 100081)
- Keywords:
- Zoysia; transcription factor; DREB; abiotic stress
- PACS:
- -
- DOI:
- 10.11937/bfyy.20180246
- Abstract:
- Zoysia is a type of warm season turf grass and shows high tolerance to multiple stresses except cold.A new dehydration-responsive element binding (DREB) gene,designated as ZjDREB1.2 (GenBank accession No:KP696367.1),was isolated from three cultivars of zoysiagrass,‘Meyer’ (Zoysia japonica),‘Jiaodongqing’ (Z.japonica) and ‘Manila’ (Z.matrella).The results showed that the open reading frame of ZjDREB1.2 was 702 bp in length,encoding 233 amino acids.The relative molecular mass of ZjDREB1.2 protein was predicted to be 25.2 kDa with a theoretical isoelectric point of 4.91.The protein possesses an incomplete AP2/ERF DNA-binding domain,which was flanked up- and down-stream directly by two signature sequences of DREB1 genes.Phylogenetic tree analysis indicated that ZjDREB1.2 protein belonged to the A-1 group of DREB subfamily and was a close relative of the DREB1 homologous of C4 plants.In the cultivar ‘Meyer’,the transcript of ZjDREB1.2 gene was up-regulated under low temperature,high salinity and drought stresses during the 2-12 hours,with the greatest response to cold,and then down-regulated to the normal around or even lower level after 72 hours.Compared with Zoysia matrella,Zoysia japonica showed a relative higher transcript level of ZjDREB1.2 at the 72 hours cold stress point.The yeast one-hybrid assay indicated that ZjDREB1.2 protein showed a strong transcriptional activity but no DRE-element binding activity.The evolution of DREB1 gene in warm season plants with incomplete AP2 domain and its function is worthy of further research.
Last Update: 2018-10-24