Abstract

Info

Title:: Optimization of Technology of Microwave Extraction of Ganoderma lucidum Polysaccharides and Its Antibacterial Activity

Author(s):: JIA Shaojie1; XIE Xiuchao1; 2; DENG Baiwan1; 2; PENG Hao1; 2; (1.School of Biological Science & Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723001;2.Shaanxi Provincial Engineering Research Center，Edible and Medicinal Fungi,Hanzhong,Shaanxi 723001)

Keywords:: microwave assisted; response surface analysis; Ganoderma lucidum polysaccharide; antibacterial activity

Abstract:: Based on the single factor experiment,the response surface method was used to optimize the extraction process of Ganoderma lucidum polysaccharides by the response surface method.Bacillus subtilis,Monilia albican,Staphylococcus aureus,Escherichia coli and Salmonella were used as test strains to study the antibacterial activity of Ganoderma lucidum polysaccharides.The minimum inhibitory concentration was determined by double dilution method.The results showed that microwave power was the best process of microwave power 374 W,extraction time 23 minutes,liquid material 15∶1 mL·g-1,extraction times 2 times,under this condition,the extraction rate of Ganoderma lucidum polysaccharides reached 4.62%.Ganoderma lucidum polysaccharides showed inhibitory effects on five kinds of tested bacteria,of which Staphylococcus aureus,Escherichia coli and Bacillus subtilis significantly inhibited the maximum inhibitory rate reached 52.4%.The minimum inhibitory concentration of Ganoderma lucidum polysaccharide against Staphylococcus aureus was 6.25 mg·mL-1,and the minimum inhibitory concentration against Bacillus subtilis and Escherichia coli was 12.5 mg·mL-1.The minimum inhibitory concentration against Monilia albican was 25.00 mg·mL-1 and the minimum inhibitory concentration against Salmonella was 50.00 mg·mL-1.