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Cloning of SsKN1 Gene From Sinningia speciosa and Its Expression in Different Tissues

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年18
Page:
69-74
Research Field:
Publishing date:

Info

Title:
Cloning of SsKN1 Gene From Sinningia speciosa and Its Expression in Different Tissues
Author(s):
BI Chunxiao; LIU Fengjuan; QU Ruihong; HU Xin; XU Quanle
(College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100)
Keywords:
Sinningia speciosa; SsKN1gene; gene cloning; tissue expression analysis
PACS:
-
DOI:
10.11937/bfyy.20180190
Abstract:
Sinningia speciosa were used as materials to clone SsKN1 gene via RT-PCR from shoot tips.Blast and phylogenetic trees via neighbor-joining (NJ) method were used to analysis the sequence.And then,semi-quantitative RT-PCR was used to investigate the different expression level of SsKN1 gene in different tissues of root,calyx,petal,stem,shoot tips and leaf.The results showed that 451 bp cDNA in length of SsKN1 gene was isolated,which shared a high identity with NTH15 and STM,and SsKN1 was not expressed in petal and stem while highly expressed in shoot tips and stem and relatively lower expressed in root and calyx.

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Last Update: 2018-10-24