Cloning and Expression Analysis of CsDREB1 in Cucumber Under Propamocarb Stress
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2018年16
- Page:
- 19-28
- Research Field:
- Publishing date:
Info
- Title:
- Cloning and Expression Analysis of CsDREB1 in Cucumber Under Propamocarb Stress
- Author(s):
- YANG Shuxin1; 2; ZHOU Xiuyan2; XIN Ming2; LIU Dong2; QIN Zhiwei2
- (1.Key Laboratory of Biology and Germplasm Creation for Horticultural Crops,Ministry of Agriculture,Harbin,Heilongjiang 150030;2.College of Horticulture,Northeast Agricultural University,Harbin,Heilongjiang 150030)
- Keywords:
- cucumber（Cucumis sativus L.）; CsDREB1; propamocarb; expression analysis
- PACS:
- -
- DOI:
- 10.11937/bfyy.20180184
- Abstract:
- High residue cucumber ‘D9320’ and low residue cucumber ‘D0351’ were selected as materials in this study,by PCR cloning method,the full length of cucumber CsDREB1 gene was obtained which could response to propamocarb,the gene characteristics and expression were analysed in order to provide reference for breeding of low residue cucumber variety.The results showed that CsDREB1 with 603 bp in cucumber and CmDREB1D in melon both came from the same small evolutionary branches,with the closest relationship by the analysis of phylogenetic tree.Promoter sequence analysis showed that CsDREB1 promoter region contained 5UTR Py-rich stretch which was a high transcription level cis-acting element,O2-site protein which could regulate zein metabolism and a MeJA responsiveness cis-acting regulatory element called CGTCA-motif.RT-PCR results showed that the expression of CsDREB1 in low residue cucumber ‘D0351’ decreased significantly under propamocarb stress compared to control,at the same time significant difference in high residue cucumber ‘D9320’ between stress and control was not found.The analysis of tissue specific expression showed that CsDREB1 was mainly expressed in the fruit,the protein encoded by CsDREB1 was located on the nucleus which belonged to the nucleoprotein.CsDREB1 negative response to propamocarb stress.Overexpression CsDREB1 in Arabidopsis under propamocarb stress test showed that the resistance ability to propamocarb of transgenic Arabidopsis significantly decreased compared to wild Arabidopsis.CsDREB1 could not respond to ABA,drought and high salt stress,neither does Corynespora cassiicola，but CsDREB1 could respond to JA.
Last Update: 2018-09-21