Abstract

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Author(s):: LI Lei1; YAN Min2; ZHAO Jia3; NIE Yuanjun4; (1.Gardening Research Institute,Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030031；2.Institute of Agriculture Environment and Resource Research,Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030031;3.Biotechnology Research Center,Shanxi Academy of Agriculture Sciences,Taiyuan,Shanxi 030031;4.Institute of Agricultural Resources and Economics,Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030031)

Keywords:: compost; fermentation; bacterial community; high throughput sequencing

Abstract:: To provide the basis for orchard branches waste resource recycling,the branches and fresh manure were used as test materials.The second generation of high-throughput sequencing technologies was used to analysis the bacterial community variation of the compost that adding exogenous starter.The results showed that total 722 931 high quality sequences were got from 12 samples.Sequencing depth could really reflect the sampling of the bacterial community diversity.Significant difference was found in bacterial community structure at different times of the composting and putrefaction.Base on the diversity index and heat map of genera analysis,fermenting agents had minimum bacteria community diversity and genera.The compost′s bacterial community was changed because of the fermenting agents,the Bacillus and Actinomycetes had become advantage groups during the composting and putrefaction,this would promote the growth of plant in future.