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Title:: Genetic Diversity of Rhododendron simsii Planch.Populations Sampled From Different Slope Aspects With ISSR Markers

Author(s):: ZHANG Beilin; LI Zhiliang; FANG Jiao; GUO Xudong; GAN Jianping; WANG Shuzhen; (Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization/Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains/College of Life Science,Huanggang Normal University,Huanggang,Hubei 438000)

Keywords:: Rhododendron simsii Planch.; genetic diversity; ISSR; slope aspect; accompanying species

Abstract:: In this study,ten ISSR markers were selected to investigate the genetic diversity of three Rhododendron simsii Planch.populations,which were sampled at different slope aspects of Huangshizhai (Luotian country).The results showed that these markers gave out 38 loci,among which 36 bands (94.74%) were polymorphic.Moreover,the number of locus ranged from 3 to 5.In particular,the percentage of polymorphic loci,Nei′s gene diversity (h),Shannon information index (I) were all the highest in the ‘Southern population’,while the lowest in the ‘Western population’.The geographical environment and accompanying species on the southern slope might showed great effect to genetic diversity of R.simsii population.The total genetic variation (Ht) was 0.268 9.AMOVA analysis showed that only 6.01% genetic variation was maintained between populations,while 93.99% was maintained within populations.The genetic identify was the highest between ‘Western population’ and ‘Northern population’.Furthermore,genetic distance was the highest between ‘Northern population’ and ‘Southern population’.This research would provide critical theoretical basis for the biological protection,sustainable development and utilization of Rhododendron resources.