|Table of Contents|

Identification and Characterization of CIPK Family Genes in Watermelon

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年08
Page:
1-7
Research Field:
Publishing date:

Info

Title:
Identification and Characterization of CIPK Family Genes in Watermelon
Author(s):
LUAN Feishi; LYU Huiling; ZHU Zicheng; ZHANG Chen; LIN Fan; HAN Qiankun
(College of Horticulture and Landscape Architecture,Northeast Agricultural University/Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast Region),Ministry of Agriculture,Harbin,Heilongjiang 150030)
Keywords:
watermelon; CIPK gene family; sequence characterization; phylogeny analysis
PACS:
-
DOI:
10.11937/bfyy.20174641
Abstract:
Calcineurin B-like-interacting protein kinase (CIPK) is one kind of kinases,which are very important for plant in responding environmental stress and the progress of development.To reveal the vital role of CIPK gene family in watermelon growth,development and anti-defense mechanism,in our study,based on watermelon genome database,the CIPK family genes structure,positions on chromosome,phylogenetic relationship,physical and chemical properties were analyzed by bioinformatics tools.The results showed that 19 kinds of ClCIPK genes were identified from watermelon whole genome,they were unevenly distributed in the chromosomes No1-6,8,10,respectively.The putative encoded proteins length of ClCIPK ranged from 330 to 565 bp.Based on gene structures,NAF domains was found in 18 of ClCIPK family genes but not in ClCIPK9.All ClCIPK genes were divided into exon-rich and exon-poor two groups;and phylogenetic analysis revealed that all the ClCIPK proteins were divided into five major clades,and every clades contained a part of CIPK genes from watermelon and arabidopsis,which indicated that the ClCIPK family had a high homology relation with AtCIPK.Based on these results,we speculated that ClCIPK genes might involve in multiple biological processes,and our research would provide a theoretical basis for the functional dissection and utilization of CIPK genes in watermelon breeding.

References:

 

[1]LUAN S.The CBL-CIPK network in plant calcium signaling[J].Trends in Plant Science,2009,14(1):37.

[2]ALBRECHT V,RITZ O,LINDER S,et al.The NAF domain defines a novel protein-protein interaction module conserved in Ca2+ regulated kinases[J].Embo Journal,2001,20(5):1051.

[3]KOLUKISAOGLU U,WEINI S,BLAZEVIC D,et al.Calcium sensors and their interacting protein kinases:Genomics of the Arabidopsis and rice CBL-CIPK signaling networks[J].Plant physiology,2004,134(1):43.

[4]ZHANG H,YANG B,LIU W Z,et al.Identification and characterization of CBL and CIPK gene families in canola(Brassica napus L.)[J].BMC Plant Biology,2014,14(1):8.

[5]TRIPATHI V,SYED N,LAXMI A,et al.Role of CIPK6 in root growth and auxin transport[J].Plant Signaling & Behavior,2009,4(7):663-665.

[6]MAO J,NURUZZAMAN M S M,SHI S,et al.Mechanisms and physiological roles of the CBL-CIPK networking system in Arabidopsis thaliana[J].Genes,2016,7(9):62.

[7]XIANG Y,HUANG Y,XIONG L.Characterization of stress-responsive CIPK genes in rice for stress tolerance improvement[J].Plant Physiology,2007,144(3):1416.

[8]ZHANG C,GE R,ZHANG J,et al.Identification and expression analysis of a novel HbCIPK2-interacting ferredoxin from halophyte H.brevisubulatum[J].PLoS One,2015,10(12):e0144132.

[9]沈金秋,郑仲仲,潘伟槐,等.植物CBL-CIPK信号系统的功能及其作用机理[J].植物生理学报,2014(5):641-650.

[10]DENG X,ZHOU S,HU W,et al.Ectopic expression of wheat TaCIPK14,encoding a calcineurin B-like protein-interacting protein kinase,confers salinity and cold tolerance in tobacco[J].Physiologia Plantarum,2013,149(3):367.

[11]余爱丽,赵晋锋,王高鸿,等.两个谷子CIPK基因在非生物逆境胁迫下的表达分析[J].作物学报,2016,42(2):295-302.

[12]刘思源,黄海锋,王旭东,等.辣椒全基因组中CBL、CIPK基因家族的鉴定及特性分析[J].分子植物育种,2017(8):2977-2985.

[13]凌秋平,曾巧英,胡斐,等.甘蔗[STBX]CIPK23[STBZ]基因克隆及对低钾、干旱、 盐胁迫的响应[J].分子植物育种,2015(6):1329-1335.

[14]朱强龙,赵玉龙,吕慧玲,等.西瓜CBL家族基因的鉴定与特征分析[J].北方园艺,2017(15):18-24.

[15]张志忠.西瓜抗病基因工程研究进展[J].农学学报,2014,4(1):85-89.

[16]章照停.西瓜优质高产栽培技术[J].上海蔬菜,2014(3):82-83.

[17]ARTIMO P,JONNALAGEDDA M,ARNOLDK,et al.ExPASy:SIB bioinformatics resource portal[J].Nucleic Acids Research,2012,40(Web Server issue):W597.

[18]CHEN A X,GU B Z,XIN B D,et al.Identification and characterization of putative CIPK genes in maize[J].Journal of Genetics and Genomics,2011,38(2):77-87.

[19]KANWAR P,SANYAL S K,TOKAS L,et al.Comprehensive structural,interaction and expression analysis of CBL and CIPK complement during abiotic stresses and development in rice[J].Cell Calcium,2014,56(2):81.

[20]YU Y,XIA X,YIN W,et al.Comparative genomic analysis of CIPK gene family in Arabidopsis and Populus[J].Plant Growth Regulation,2007,52(2):101-110.

[21]HU W,XIA Z,YAN Y,et al.Genome-wide gene phylogeny of CIPK family in cassava and expression analysis of partial drought-induced genes[J].Front Plant Sci,2015,30(6):914.

[22]ROYS W,PENNY D.Patterns of intron loss and gain in plants:Intron loss-dominated evolution and genome-wide comparison of O.sativa and A.thaliana[J].Molecular Biology & Evolution,2007,24(1):171.

[23]NURUZZAMAN M,MANIMEKALAI R,SHARONI A M,et al.Genome-wide analysis of NAC transcription factor family in rice[J].Gene,2010,465(1-2):30.

[24]THODAY-KENNEDY E L,JACOBS A K,ROY S J.The role of the CBL-CIPK calcium signalling network in regulating ion transport in response to abiotic stress[J].Plant Growth Regulation,2015,76(1):3-12.

[25]BATISTIC O,KUDLA J.Integration and channeling of calcium signaling through the CBL calcium sensor/CIPK protein kinase network[J].Planta,2004,219(6):915-924.

[26]许园园,李晓刚,李慧,等.梨CDPK基因家族全基因组序列鉴定分析[J].江苏农业学报,2015(3):659-666.

[27]LI J,LONG Y,QIG N,et al.The Os-AKT1 channel is critical for K+ uptake in rice roots and is modulated by the rice CBL1-CIPK23 complex[J].Plant Cell,2014，26(8):3387-3402.

Memo

Memo:
-
Last Update: 2018-04-27