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Title:: Optimization of Microwave-assisted Extraction of Laccaria laccata Polysaccharides by Response Surface Method and Its Antioxidant-activities

Author(s):: LI Li; HE Jianmin; LIU Yan; ZHANG Dingli; QIAN Yonglin; (Department of Resource and Environment,Baoshan College,Baoshan,Yunnan 678000)

Keywords:: microwave-assisted; Laccaria laccata; response surface method; polysaccharides

Abstract:: The wild Laccaria laccata in Baoshan was used as the material,the polysaccharides was extracted by microwave assisted method,based on the single factor experiment,the response surface method of Box-Behnken significant factors of microwave time,microwave power and liquid-solid ratio.The antioxidation of polysaccharides was determined by the scavenging effect of DPPH· and ·OH.The results showed that the optimum conditions of Laccaria laccata polysaccharides were：microwave time 90 seconds,microwave power 350 W,liquid-solid ratio 50∶1 mL·g-1,water bath temperature 80 ℃,water bath time 2 hours,and the polysaccharide yield was 6.75%.Compared with predicted value (6.79%),it had a smaller relative error at 0.59%.Furthermore,Laccaria laccata polysaccharides had remarkable scavenging activity to DPPH· and ·OH,which indicated that was a strong natural antioxidation and further study was worth doing.