|Table of Contents|

Isolation, Identification and Water-retaining Effect of ZMN-3 From Desertified Soil

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年08
Page:
3-
Research Field:
Publishing date:

Info

Title:
Isolation, Identification and Water-retaining Effect of ZMN-3 From Desertified Soil
Author(s):
REN Mengnan 1; JIN Yu 1; LI Xiaoyan 2; LI Siqi 1; WANG Yanmei 1; Huang Haidong 1
1.College of Agronomy and Resources and Environment, Tianjin Agricultural University, Tianjin 300384; 2.Department of Food Science and Biotechnology, Tianjin Agricultural University, Tianjin 300384
Keywords:
extracellular polysaccharide;  Massilia;  identification;  water retention effect
PACS:
-
DOI:
10.11937/bfyy.20174354
Abstract:
Desertified soil was used as experimental material. Polysaccharides synthetic strain ZMN-3 was isolated and identified by the method of dilution flat and polyphasic taxonomy. For the purpose of supply bacteria resources and reference for applying in artificial biological crust fix sand, properties of fermentation broth and water conservation of strain ZMN-3 were investigated. The results showed that strain ZMN-3 belonged to the genes of Massilia, exhibiting the highest 16S rRNA sequence similarity with Massilia plicata 76T(96.5%). On the basis of phylogenetics, physiological and biochemical analysis, strain ZMN-3 was considered to represent a novel species of the genus Massilia. The extracellular polysaccharide synthesized by ZMN-3 consisted of two components with different molecular weight distribution. The weight average molecular weight Mw was 1.91×104 kDa and 947 kDa, respectively. After incubation at 30 ℃ for 3 days, the viscosity of ZMN-3 fermentation broth reached 2 150 mPa?s, as viscous pseudoplastic fluid. The microbial agent of ZMN-3 was sprayed on the surface of sand, compact bio-crust formed and the ability of water conservation of sand increased.

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Last Update: 2018-03-01