Bioinformatics Analysis and Expression Analysis of AtOFP8 in Arabidopsis thaliana(PDF)
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2018年18
- Page:
- 35-41
- Research Field:
- Publishing date:
Info
- Title:
- Bioinformatics Analysis and Expression Analysis of AtOFP8 in Arabidopsis thaliana
- Author(s):
- TANG Yao1; ZHANG Wei1; 2; YIN Yanli1; FENG Peng1; CHEN Yufeng1; CHANG Ying1
- (1.College of Life Sciences,Northeast Agricultural University,Harbin,Heilongjiang 150030;2.Keshan Branch,Heilongjiang Academy of Agricultural Sciences,Keshan,Heilongjiang 160000)
- Keywords:
- Arabidopsis thaliana; transcription factor; AtOFP8; bioinformatics
- PACS:
- -
- DOI:
- 10.11937/bfyy.20174346
- Abstract:
- The Columbia-0 Arabidopsis thaliana was used as material.Bioinformatics analysis of Arabidopsis transcription factor AtOFP8 was carried out by using bioinformatics methods and [STBX]AtOFP8[STBZ] gene expression analysis was used by fluorescence quantitative PCR technology.Both of them laid the foundation for further research on the function of other AtOFPs.The results showed that the full-length of [STBX]AtOFP8[STBZ] gene was 954 bp,AtOFP8 protein was composed of 221 amino acids,its molecular weight was 25.466 kDa,it had no transmembrane domain and signal peptide,it was located in the nucleus and it had strong hydrophilicity.In addition,[STBX]AtOFP8[STBZ] gene was highly expressed in roots and flowers,and its transcriptional level was obviously elevated in the environment of high temperature and drought.
Last Update: 2018-10-16