Abstract

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Title:: Induction of Callus of Stem Segments of Lonicera japonica Thunb.var.chinensis (Wats.) Bak

Author(s):: HENG Jing1; HAN Chunye1; WANG Mingshan1; MA Li2; (1.Henan Vocational College of Agriculture,Zhengzhou,Henan 451450;2.Shangqiu Normal University,Shangqiu,Henan 476000)

Keywords:: Lonicera japonica Thunb.var.chinensis (Wats.) Bak; stem segment; callus; tissue culture

Abstract:: The stem of Lonicera japonica Thunb.var.chinensis (Wats.) Bak was taken as experimental materials,the factors affecting the callus induction and proliferation of Lonicera japonica Thunb.var.chinensis (Wats.) Bak were studied by orthogonal design.The results showed that 6-BA and KT had obviously promoted callus induction and increased the rate of callus induction.It was the most beneficial to the proliferation of callus when the concentration NAA and KT were 0.4 mg·L-1 and 1.0 mg·L-1,respectively.The effect of callus induction was different in different seasons,and the stem segments collected in spring were more favorable for callus induction,and it was better to use 6.5 mg·L-1 agar.The callus induction medium was suitable for MS+KT 1.0 mg·L-1+NAA 0.2 mg·L-1+6-BA 1.5 mg·L-1.The optimum medium for callus proliferation was MS+6-BA 1.5 mg·L-1+NAA 0.4 mg·L-1+KT 0.1 mg·L-1.