|Table of Contents|

Identification of Herba eupaatorii Fortunei Powdery Mildew

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年09
Page:
77-80
Research Field:
Publishing date:

Info

Title:
Identification of Herba eupaatorii Fortunei Powdery Mildew
Author(s):
DING Jinping; HOU Ying; ZHANG Qingchen; PEI Dongli
(Key Laboratory of Plant-Microbe Interactions,Shangqiu Normal University,Shangqiu,Henan 476000)
Keywords:
Herba eupaatorii Fortunei; Podosphaera fusca; ITS sequences; molecular systematic identification
PACS:
-
DOI:
10.11937/bfyy.20174286
Abstract:
Herba eupaatorii Fortunei leaves infected by the powdery mildews were used as materials.The pathogen of H.eupaatorii Fortunei powdery mildew was analyzed by morphological,etiological and molecular systematic identification.In order to make clear the phylogenetic evolution relationships of powdery mildew detected in Herba eupaatorii Fortunei in Shangqiu of Henan in China and supply reference for its control.The results showed that the primary conidia of the fungus were ovality or subspherical,measuring (18-27)μm×(15-22)μm.Long unbranched germ tube was unilateral lateral germination from the ends of conidia.Conidiophores was cylindrical,simple unbranched,composed of a basal cell with swollen base and 3 to 6 barrel-shaped conidia formed in chains,measuring (112-180)μm×(9-12)μm.Mycelial appressorium showed a nipple-shaped.Chasmothecia were not observed in the collected samples.Pathogenicity of inoculated leaves had no differences with leaves in natural station of amorphous dirt white patch.Internal transcribed spacer (ITS) sequence of its ribosomal DNA was amplified by PCR and sequenced to obtain the 565 bp target fragment (GenBank number:JX546297),comparison with sequences in the GenBank database revealed that the ITS sequence showed 100% homology with the sequence of Podosphaera fusca on Calendula officinalis (Accession No.AB525914) and Syneilesis palmata (Accession No.AB040349).The ITS sequence analysis further verified that the causal agent of the powdery mildew on H.eupaatorii Fortunei was Podosphaera fusca.

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Last Update: 2018-05-23