Abstract

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Author(s):: SONG Shunhua1; WU Ping1; XU Mao2; GENG Lihua1; MENG Shuchun1; (1.Beijing Vegetable Research Center,Beijing Academy of Agricultural and Forestry Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of North China Area/Beijing Key Laboratory of Vegetable Germplasm Improvement,Ministry of Agriculture,Beijing 100097;2.Beijing Shunyi District Planting Center，Beijing 101300)

Abstract:: Typical diseased leaf and fruit samples of watermelon with suspected virus-like symptoms from Shunyi,Beijing were used as experimental materials.Virus detection for leaf and four positions of fruits (rind,pulp,pedicel and fruit stem),was carried out by reverse transcript polymerase chain reaction (RT-PCR).The aim was to understand the species infecting watermelon and provide reference for control of watermelon virus diseases.The results showed that three viruses (WMV-2,ZYMV,CMV) were found in total of 11 viruses detected.WMV-2 was found in all of five parts,ZYMV in rind,pulp and fruit stem,CMV in pulp and pedicel.Three viruses were found in different parts of the same fruit.The results indicated that the virus in fruit were mix-infected.WMV-2 was found in leaf and fruit,implying that it was the virus dominant specie in Shunyi region,Beijing.