Ultrasonic-assisted Extraction and Antioxidant Activity of Polysaccharides From Laccaria laccata
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2018年17
- Page:
- 133-138
- Research Field:
- Publishing date:
Info
- Title:
- Ultrasonic-assisted Extraction and Antioxidant Activity of Polysaccharides From Laccaria laccata
- Author(s):
- ZHANG Shuihua1; LI Benhong2; ZHOU Weidong1
- (1.Faculty of Chemistry and Environmental Science,Qujing Normal College,Qujing,Yunnan 655011；2.Library,Qujing Normal College,Qujing,Yunnan 655011)
- Keywords:
- Laccaria laccata; polysaccharides; ultrasonic; DPPH·; ABTS+·
- PACS:
- -
- DOI:
- 10.11937/bfyy.20174106
- Abstract:
- Taking Laccaria laccata as the raw material,the effects of extraction conditions such as ratio of liquid-solid,ultrasonic time,ultrasonic power,ultrasonic temperature and time of extraction were investigated by one-factor tests and orthogonal test,and the extraction polysaccharides were analyzed with DPPH· and ABTS+· test.The results showed that the times of extraction affected the yield markably,the second was ratio of liquid-solid,and ultrasonic time had the least influence;the optimum coditions were determined as follows:times of extraction of 3 times,ratio of liquid-solid of 20∶1 mL·g-1,ultrasonic power of 300 W,ultrasonic temperature of 70 ℃ and ultrasonic time of 40 minutes.Under the condition,the yield was up to 6.27%.Polysaccharides exhibited antioxidanteffects in 2,2′-diphenyl-1-picrylhydrazyl(DPPH·),2,2′-azino-bis(3-ethylben-othiazoline-6-sulfonic acid(ABTS+·) radical scavenging assay.And it showed that the extraction polysaccharides which had some oxidation resistance.
Last Update: 2018-09-30