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Title:: Effects of Salt Stress on Stipa breviflora Seedling and Antioxidant Isozyme

Author(s):: WANG Jing; ZI Hongzhou; LI Huangwei; NING Yanxia; LUO Ling; (College of Biological Science and Engineering,North Minzu University,Yinchuan,Ningxia 750021)

Abstract:: Stipa breviflora seeds,collected from Inner Mongolia,were sowed on MS medium containing 0,50,100,150,200 mmol·L-1NaCl,to investigate the effect of salt stress on the physiological phenotype and antioxidant enzymes activity of Stipa breviflora seedling.The results showed the germination rate,root length and fresh weight of seedling [JP2]were all influenced by salinity.Germination rate,root length and fresh weight of seedling were significantly inhibited by 100,150 and 50 mmol·L-1 NaCl and data were 62%,1.30 cm,0.299 g respectively.With NaCl content increasing,the tread of superoxide dismutase (SOD) and peroxidase (POD) activity were first rising and then falling.When the NaCl concentration reached 150 and 100 mmol·L-1,the activity of SOD and POD were the maximum,48.6 U·g-1 and 6 766 U·g-1 respectively.The activity of catalase (CAT) was continuing increased and reached the maximum at 100 mmol·L-1,was 299 U·g-1.The isozyme showed SOD 2-4,POD 1-2,and CAT 1-2 were positively correlated with salinity tolerance of Stipa breviflora seedling.