Application of Two Bacteriostatic Agents in Open Tissue Culture of? Dendrobium officinale
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2018年12
- Page:
- 136-140
- Research Field:
- Publishing date:
Info
- Title:
- Application of Two Bacteriostatic Agents in Open Tissue Culture of? Dendrobium officinale
- Author(s):
- HUANG Min; LIANG Chunhui; LI Xiuping; LI Rongxi; TANG Huimin
- (Department of Tropical Crops,Guangdong AIB Polytechnic College,Guangzhou,Guangdong 510507)
- Keywords:
- bacteriostatic agents; Dendrobium officinale; open tissue culture; application
- PACS:
- -
- DOI:
- 10.11937/bfyy.20174048
- Abstract:
- In order to reduce the cost of industrialized breeding of Dendrobium officinale,simplify the process of tissue culture and increase economic efficiency,the suitable antibacterial agent formula for open tissue culture of Dendrobium candidum was explored.In this study,Dendrobium candidum was used as the test material,different concentrations of sodium hypochlorite（0%-0.2%） and mancozeb（0%-0.03%）as bacteriostatic agent were added to the culture medium,traditional tissue culture as a control,the optimal concentration of two antimicrobial agents in open tissue culture of Dendrobium candidum was explored.The results showed that sodium hypochlorite and mancozeb could be used as antibacterial agents in open tissue culture of Dendrobium officinale.It demonstrated that the effective concentration of sodium hypochlorite was above 0.025%,the effective concentration of mancozeb was above 0.006% on reducing the pollution of the culture medium and the culture environment.When the concentration of sodium hypochlorite was above 0.200% and mancozeb concentration above 0.024% could completely inhibit the pollution caused by innoculated bacteria and fungi.The results showed that the multiplication coefficient of Dendrobium officinale in the open tissue culture were 2.48 at the concentration of 0.150% sodium hypochlorite and the multiplication coefficient were 2.68 at the concentration of 0.020% mancozeb.There was no significant difference between the two test groups and control group.Therefore,the two kinds of bacteriostatic agents had good application value in the open tissue culture of Dendrobium officinale.
Last Update: 2018-07-27