Abstract

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Title:: Biological Characteristics of Fusarium oxysporium(Schl.) f.sp.cucumerinum Owen

Author(s):: KANG Pingzhi; BAI Xiaojun; ZHANG Lirong; DU Yuning; ZHANG Zhike; (Institute of Plant Protection，Ningxia Academy of Agriculture and Forestry Sciences/Ningxia Key Laboratory of Plant Disease and Pest Control,Yinchuan,Ningxia 750002)

Keywords:: cucumber wilt; Fusarium oxysporium(Schl.) f.sp.cucumerinum Owen; biological characteristics

Abstract:: Fusarium oxysporium f.sp.cucumerinum(FOC) was used as test material,the effects of different temperature，pH，illumination，medium，sources of carbon and nitrogen and trace elements on mycelium growth，spores production and spores germination of FOC were studied using methods of mycelial growth rate and cell count technique in order to make clear its optimum growth conditions.It provided a reference for the prevention and control of the cucumber wilt in the field.The results showed that the fitting range of temperature of FOC was between 20-30 ℃,the optimum value was 25 ℃.The pH 6.0-9.0 were the most suitable for FOC,and germination rate of spores was higher at pH 7.0-9.0.The alternation of light and darkness and total dark conditions were more beneficial for mycelium growth,spores production and spores germination for FOC.The growth rate of FOC in PDA and PSA medium were faster,and its quantity of spore and germination rate of spore were also higher,followed by PMA and OA mediums.Its growth rate,quantity of spores and germination rate of spores were the best in the medium containing glucose,maltose,peptone potassium nitrate,sodium nitrate and green vitriol.