|Table of Contents|

Optimization of SSR-PCR Reaction System for Apple Callus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年10
Page:
53-56
Research Field:
Publishing date:

Info

Title:
Optimization of SSR-PCR Reaction System for Apple Callus
Author(s):
GAO Bing1; 2; AN Wenjie1; SUN Jun2; 3
(1.Department of Horticulture,Shanxi Forestry Vocational and Technical College,Taiyuan,Shanxi 030009；2.College of Horticulture,Nanjing Agricultural University,Nanjing，Jiangsu 210095;3.College of Horticulture,Anhui Agricultural University,Hefei，Anhui 230036)
Keywords:
apple;  callus;  SSR-PCR;  system optimization
PACS:
-
DOI:
10.11937/bfyy.20173431
Abstract:
In order to establish the basis of genetic variation research of apple callus，the ‘Gala’ induction of callus was DNA template extraction materials.the Mg2+，Taq DNA polymerase，primer dosage，template DNA concentration and dNTP which were suitable for concentration or dosage were explored.The results showed that the effect of different levels on reaction system was Taq DNA polymerase>dNTP dosage>Mg2+ dosage>primer dosage=template DNA concentration.The optimal reaction system of apple callus was established,the optimal reaction system of apple callus was that 25 μL for total volume，which were 4 μL 25 mmol?L-1 Mg2+，0.25 μL 5 U?μL-1Taq DNA polymerase，3 μL 0.01 mmol?L-1 SSR，1 μL 30 ng?μL-1 template DNA，4 μL 2.5 mmol?L-1dNTP，2.5 μL 10×PCR Buffer，10.25 μL ultra-pure water.

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Last Update: 2018-06-01