|Table of Contents|

Expression Analysis and Construction of Plant Expression Vector of  AmWRKY53 in Ammopiptanthus mongolicus

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年06
Page:
1-9
Research Field:
Publishing date:

Info

Title:
Expression Analysis and Construction of Plant Expression Vector of  AmWRKY53 in Ammopiptanthus mongolicus
Author(s):
XIA Bolin; XING Yide; GAO Fei; JIANG Chengyong; ZHOU Yijun
(College of Life & Environmental Sciences,Minzu University of China,Beijing 100081)
Keywords:
Ammopiptanthus mongolicus; AmWRKY53; expression patterns; construction of plant expression vector
PACS:
-
DOI:
10.11937/bfyy.20173341
Abstract:
In this study,a gene encoding WRKY transcription factor was cloned from Ammopiptanthus mongolicus,and it was named as AmWRKY53.The sequence contained an open reading frame (ORF) of 1 065 bp,which encoded peptide with 354 amino acids.There were one WRKYGQK domain and one C2HC zinc finger motif in AmWRKY53〖STBZ〗,so it belonged to type Ⅲ WRKY transcription factor.Quantitative real-time PCR (qRT-PCR) analyses revealed that the AmWRKY53 was induced by drought and high salt stress.The pPZP212-[STBX]AmWRKY53 expression vector was constructed and laid the foundation for further study of AmWRKY53.

References:

 

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Last Update: 2018-03-30