|Table of Contents|

Optimization of Protoplast Preparation and Regeneration Conditions of  Hericium erinareus Fusion Breeding

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年09
Page:
159-165
Research Field:
Publishing date:

Info

Title:
Optimization of Protoplast Preparation and Regeneration Conditions of  Hericium erinareus Fusion Breeding
Author(s):
YANG Shan1; 2; ZHANG Henan1; LI Qiaozhen1; WU Di1; CHEN Mingjie1; YANG Yan1
(1.Key Laboratory of Edible Fungus Resources and Utilization (South),Ministry of Agriculture/National Engineering Research Center of Edible Fungi/Institute of Edible Fungi，Shanghai Academy of Agricultural Sciences，Shanghai 201403；2.Food College,Shanghai Ocean University，Shanghai 201306)
Keywords:
Hericium erinareus; protoplast; preparation; regeneration rate
PACS:
-
DOI:
10.11937/bfyy.20173296
Abstract:
Hericium erinareus 0605 was used as test material，the influence of enzyme system，enzymolysis time，enzymolysis temperature，mycelial age and shaking speed on the preparation and regeneration of protoplasts of Hericium erinareus 0605 were studied by single factors and orthogonal design experiments.The results showed that the optimal preparing and regeneration conditions of Hericium erinareus protoplasts were mycelium age 10 days，using 2.0% lywallzyme and 0.5% driselase，digesting at 32 ℃，shaking speed 120 r?min-1 for 2.5 hours.Under this conditions，the study established the foundation of protoplast fusion breeding of Hericium erinareus.

References:

 

[1]吴云艳,宋慧,马福.猴头菌属(Hericium)真菌多糖的研究进展[J].吉林农业大学学报,2005,27(6):621-626.

[2]袁胜东,罗霞,余梦瑶,等.猴头菌生物活性成分及药理作用研究进展[J].安徽农业科学,2011,39(15):8926-8928.

[3]李巧珍,唐传红,杨焱,等.猴头菌活性多糖高产菌株的筛选[J].食用菌学报,2012(2):66-68.

[4]谭琦,潘迎捷,汪昭月,等.用单核原生质体杂交育成香菇新菌株申香8号[J].食用菌学报,1999,6(2):1-4．

[5]周伏忠,贾身茂,李秀玉.食用菌原生质体巨大化及其再生的初步观察[J].微生物学通报,1996,23(2):69-74.

[6]李蕤,马守能,刘群.香菇菌丝原生质体制备及融合条件的研究[J].安徽大学学报(自然科学版),2001,25(3):99-102．

[7]李明.原生质体紫外诱变选育灵芝新菌种的研究[J].微生物学报,2001,41(2):229-233.

[8]罗雯,陈志勤.微生物原生质体融合技术及其在育种中的应用[J].西安联合大学学报,2003,6(4):5-9.

[9]张渊,王谦,张筱梅,等.茶薪菇原生质体制备及诱变的研究[J].中国食用菌,2003,23(3):15-17.

[10]马向东,周伏忠,吴浩洁,等.猴头菌原生质体制备条件的研究[J].河南科学,1995(1):65-69.

[11]李艳红,李莉,潘延宁.猴头菌原生质体分离条件研究[J].微生物学杂志,2006,26(3):28-30.

[12]张琪,刘宏伟,陈娟,等.猴头菌原生质体制备及单核体鉴定研究[J].中国医药生物技术,2015,10(2):113-118.

[13]BOWMAN S M,FREE S J.The structure and synthesis of the fungal cell wall[J].Bio Essays,2006,28(8):799-808.

[14]祝子坪.桑黄菌的物理诱变与发酵研究[D].镇江:江苏大学,2007.

[15]王昱,王义,王康宇,等.灵芝原生质体的制备与再生研究[J].北方园艺,2013(16):184-188.

[16]刘红丽,郭成金.美味蘑菇原生质体制备与再生研究[J].天津农业科学,2012,18(4):146-151.

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Last Update: 2018-05-25