|Table of Contents|

Medium Optimization of Liquid Fermentation and Production of  Lignin Degradation Enzyme in Pleuratus ferulae

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年09
Page:
153-159
Research Field:
Publishing date:

Info

Title:
Medium Optimization of Liquid Fermentation and Production of  Lignin Degradation Enzyme in Pleuratus ferulae
Author(s):
ZHANG Shiqi; Tunnisa MAITISAIYIDI; LIU Hongju; TUO Fei; YANG Kailun
(College of Animal Sciences，Xinjiang Agricultural University，Urumqi，Xinjiang 830052)
Keywords:
Pleuratus ferulae; carbon source; nitrogen source; growth factor; laccase; manganese peroxidase
PACS:
-
DOI:
10.11937/bfyy.20173285
Abstract:
Pleuratus ferulae NB-1 was used as donor strain，with liquid fermentation to determine the optimum fermentation time.Besides，this experiment optimized carbon source，nitrogen source and growth factor in medium using biomass，laccase activity and the activity of manganese-dependent peroxidase as index.The results showed that the optimum incubation time of liquid fermentation in Pleuratus ferulae was 7 days.For carbon source，the contents of biomass in soluble starch group and sucrose group were significantly higher than those in glucose group and maltose group (P<0.01).The laccase activity in soluble group was significantly higher than that in other groups (P<0.01).The highest activity of manganese-dependent peroxidase was maltose group (P<0.01).When it came to nitrogen source factors，the contents of biomass in beef extract group and peptone group were remarkably higher than those in urea group and ammonium sulfate group (P<0.01).Beef extract group′s laccase activity was significantly higher than that in other three groups (P<0.01).The activity of manganese-dependent peroxidase in peptone group was minimum，and significantly lower than ammonium sulfate group，beef extract group and urea group (P<0.01).All the four growth factor group′s biomass were remarkably higher than those in control group (P<0.01)，and vitamin C group was the best.The activity of manganese-dependent peroxidase in vitamin C group was significantly higher than that in vitamin B group and control group (P<0.01).It was concluded that the best incubation time of liquid fermentation in Pleuratus ferulae was 7 days，and the optimal carbon source，nitrogen source and growth factor in medium were soluble starch，beef extract and vitamin C respectively.

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Last Update: 2018-05-25