|Table of Contents|

Mycorrhizal Microstructure of Cymbidium hybridum and Isolation and  Identification of Its Mycorrhizal Fungi

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年08
Page:
92-96
Research Field:
Publishing date:

Info

Title:
Mycorrhizal Microstructure of Cymbidium hybridum and Isolation and  Identification of Its Mycorrhizal Fungi
Author(s):
LI Xiaoliu1; 2; FAN Yongshan2; SONG Yanchao2; LI Yanmei2
(1.Tangshan Preschool Teachers College,Tangshan,Hebei 063700；2.Department of Life Science,Tangshan Normal University,Tangshan,Hebei 063000)
Keywords:
Cymbidium hybridum; mycorrhizal fungi; isolation and identification; microstructure
PACS:
-
DOI:
10.11937/bfyy.20173259
Abstract:
Pot-cultured Cymbidium hybridum was taken as experimental material,the mycorrhizal microstructure was observed with the root tissue slices,the endophytic fungi was isolated and cultivated by the tissue isolation method,and the fungi was identified through the microscopic observation of the mycelium and spore morphology.To lay a foundation in clarification of the interaction mechanism of Cymbidium hybridum and its mycorrhizal fungi.The results showed that the cortex cells were the main colonization position of mycorrhizal fungi.The mycorrhizal fungi invaded into cortex cells mainly by passage cells if the root velamina were integrated.Besides,they could invade into cortex cells by velamina cells if the root velamina were not fully integrated.And 47 fungal strains were isolated from the mycorrhizal of pot-cultured Cymbidium hybridum and 29 out of them were identified as Fusarium sp.,18 strains were Trichoderma sp.The appearance frequencies of Fusarium sp.and Trichoderma sp.were 61.7% and 38.3%,respectively,indicating that Fusarium was the dominant mycorrhizal fungus of pot-cultured Cymbidium hybridum.

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Last Update: 2018-05-03