Establishment of Tissue Culture and Rapid Propagation System of 

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: XIONG Bingquan1; TIAN Jin2; LIAO Xiangjian1; ZHENG Xuelian2; DENG Kejun2; (1.Department of Landscape Gardening,Chengdu Agricultural College,Chengdu，Sichuan 611130；2.School of Life Sciences and Technology,University of Electronic Science and Technology of China,Chengdu，Sichuan 610054)

Keywords:: succulent plant; Haworthia truncata; rapid propagation technology

Abstract:: Haworthia truncata was taken as test material,effects of selection of explants and proportion of hormone in culture medium of tissue culture of Haworthia truncata were studied.In order to optimize the tissue culture conditions of Haworthia truncata,this test established a tissue culture and rapid propagation system that was suitable for industrial seedling rearing.The transverse section of root neck of Haworthia truncata was the best as the explant in callus induction stage.The best media for each stages were as follows：the best medium for callus induction was MS+1.0 mg?L-1 6-BA+0.1 mg?L-1 NAA,the best medium for inducing stem and leaf was MS+2.0 mg?L-1 6-BA+0.1 mg?L-1 NAA,the best medium for inducting root was MS+0.1 mg?L-1 NAA.

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Last Update: 2018-04-20