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Title:: Association Analysis Between Microsatellite Loci and Phenotypic Traits in Lactuca sativa L.

Author(s):: WANG Shuzhen1; WANG Bincai2; HUANG Xingxue2; LI Zhiliang1; ZHOU Guolin2; WANG Aihua2; (1.Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization/College of Life Science,Huanggang Normal University,Huanggang,Hubei 438000;2.Wuhan Vegetable Research Institute,Wuhan Academy of Agricultural Science & Technology,Wuhan,Hubei 430065)

Keywords:: Lactuca sativa L.; trait; SSR marker; association analysis; germplasm improvement

Abstract:: Among 300 lettuce germplasms collected worldwide,30 samples were selected as materials for the survey of phenotypic variation.And 12 traits were surveyed,SSR molecular markered analysis was conducted.Association analysis between microsatellite loci and phenotypic traits were performed to provide the theoretical basis for molecular marker assisted breeding of lettuce.The results showed that significant differences existed in phenotypic traits,and the remarkable one was strain amplitude (40.256%).These phenotypic traits divided the 30 lettuce resources into two categories,with the genetic distance ranging between 0.083 and 0.667.Furthermore,each polymorphic EST-SSR marker amplified 3-8 alleles,and the mean HO and HE were 0.156 and 0.680,respectively.PIC value varied from 0.466 to 0.771.Similarly,the 14 SSR markers divided 30 resources into two categories,and genetic distance ranged from 0.032 to 2.629.The clustering results of phenotypic traits were not related to that of SSR markers.The correlation analysis between single trait and SSR marker showed that most markers were correlated with phenotypic traits.In particular,most marker combinations showed cumulative effects.Keywords:Lactuca sativa L.;trait;SSR marker;association analysis;germplasm improvement