|Table of Contents|

Study on Bacteriostasis in vitro of Gentiana rhodantha

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年07
Page:
128-132
Research Field:
Publishing date:

Info

Title:
Study on Bacteriostasis in vitro of Gentiana rhodantha
Author(s):
FANG Yumei; ZHANG Jiaman; SUN Aiqun; LU Hong
(Department of Biology Science，Liupanshui Normal University，Liupanshui,Guizhou 553004)
Keywords:
Gentiana rhodantha; ultrasonic extraction; bacteriostasis
PACS:
-
DOI:
10.11937/bfyy.20173105
Abstract:
Gentiana rhodantha was extracted from Liupanshui gentian by ultrasonic method.Filter paper diffusion method was used to detect whole grass,ground part,tissue culture plantlet root ultrasonic extract antibacterial activity on Escherichia coli,Bacillus subtilis and Staphylococcus aureus,and to detect the effect of oxidant,temperature on antibacterial activity.The results showed that the three kinds of bacteria antibacterial effect of tissue culture plantlet root>whole grass>ground part；the inhibitory effect of whole grass and tissue culture plantlet root on Bacillus subtilis>Escherichia coli>Staphylococcus aureus.The inhibitory effect of ground part on Escherichia coli>Bacillus subtilis>Staphylococcus aureus.Bacteriostatic ring diameter of Escherichia coli,Bacillus subtilis and Staphylococcus aureus when the concentration of extraction solution was 0.125 g?mL-1,the tissue culture plantlet root was （11.33±0.44）,（11.67±0.38）,（9.67±0.53）mm,and the whole grass was （9.42±0.45）,（9.05±0.72）,（8.36±0.44）mm,and the ground part was （8.34±0.42）,（6.00±0.00） and （9.33±0.44）mm.The oxidant had little effect on the antibacterial activity of G.rhodantha,and the extract of G.rhodantha had thermal stability.The roots of tissue cultured plantlets of G.rhodantha could be developed into natural bacteriostatic agents.

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Last Update: 2018-04-23