|Table of Contents|

Establishment of a Regeneration System From Leaf Explant of Stevia rebaudiana

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年06
Page:
16-22
Research Field:
Publishing date:

Info

Title:
Establishment of a Regeneration System From Leaf Explant of Stevia rebaudiana
Author(s):
BAO Han1; 2; ZHANG Rui1; 2; ZHANG Meiling1; 2; ZHANG Hong1; 2; CHEN Ren1; 2
(1.Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Ningxia University,Yinchuan,Ningxia 750021; 2.School of Life Science,Ningxia University,Yinchuan,Ningxia 750021)
Keywords:
Stevia rebaudiana;  tissue culture;  plant growth regulator; callus;  adventitious bud; cluster bud
PACS:
-
DOI:
10.11937/bfyy.20173097
Abstract:
In order to develop a rapid and efficient method for plant regeneration of Stevia rebaudiana,the segments of leaf,stem and nodalcut from the cultivar ‘Zhongshan No.2’,were used as explants,and the effects of the MS(murashige and skoog) basal medium supplied with three kinds of plant growth regulators,NAA(1-Naphthaleneacetic acid),BAP(6-Benzylaminopurine) and KT(kinetin) in different concentrations on the stages of callus and adventitious bud inducement were determined.Then the leaf was selected as optimal explant,and was cultured on the media that substituted TDZ(N-phenyl-N′-1,2,3-thidiazol-5-yl-urea) for NAA(BAP and KT remained unchanged).The results showed that the most adventitious buds were differentiated from the induced calli on the MS basal medium supplied with 3.0 mg?L-1 TDZ combined with 1.0 mg?L-1 BAP and 0.5 mg?L-1 KT,following a large number of cluster bud sappeared.The cluster buds elongated and differentiated leaves,stem and roots into a complete plantlet.With short production cycle,high propagation coefficient,the regeneration method could be used for rapid mass propagation and gene transformation.

References:

 

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Last Update: 2018-03-30