Preliminary Study on Germination Inhibitors From Ilex asprella Fruits

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Preliminary Study on Germination Inhibitors From Ilex asprella Fruits

Author(s):: ZENG Xiangda; LI Yankun; ZHUO Yinan; WANG Meiling; HE Rui; ZHAN Ruoting; (Research Center of Chinese Herbal Resource Science and Engineering,Guangzhou University of Traditional Chinese Medicine/Key Laboratory of Chinese Medicinal Resource From Lingnan,Ministry of Education/Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines,Guangzhou,Guangdong 510006)

Keywords:: Ilex asprella; germination inhibitor; systematic solvent extraction; germination rate; root length

Abstract:: Extracts of different parts (seed,flesh and peel) of fresh Ilex asprella fruits were used as research objects and Chinese cabbage seeds were taken as test material.The extracts were prepared by using systematic solvent extraction method and diluted into different concentrations,including 0.100,0.067,0.033,0.017 g?mL-1,which were applied to Chinese cabbage seeds in germination test.The effects of different extracts on germination and radicle length were compared in order to determine the types and parts of the germination inhibitors in Ilex asprella fruits.The results showed that endogenous germination inhibitors were found in the three parts of Ilex asprella fruits.Petroleum extracts and water extracts from the seeds significantly inhibited the germination of Chinese cabbage seeds；in the flesh,while there was a significant inhibiting effect on the germination rate of Chinese cabbage seeds under the treatment of petroleum extracts and methanol extracts,the ethyl acetate extracts reduced the radicle length of seedlings.Ethyl acetate,methanol and water extracts from the peels significantly inhibited the germination of Chinese cabbage seeds,and the petroleum ether,methanol and water extracts significantly reduced the radicle length of the seedlings.

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Last Update: 2018-04-23