|Table of Contents|

Effects of Different Bacterial Agents on Bacterial Diversity of Grape Leaves

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年04
Page:
82-87
Research Field:
Publishing date:

Info

Title:
Effects of Different Bacterial Agents on Bacterial Diversity of Grape Leaves
Author(s):
GUO Jiping1; MA Guang1; WANG Zhijie1; QI Shanhou1; WANG Baomei2
1.Department of Life Science,Hengshui University,Hengshui，Hebei 053000;2.Baoji Academy of Agricultural Sciences,Baoji,Shaanxi 722400
Keywords:
grape leaves; bacterial agent; bacteria diversity; PCR-DGGE
PACS:
-
DOI:
10.11937/bfyy.20172871
Abstract:
Grape leaves that were treated by Microbacterium testaceum （Y24) and Bacillus amyloliquefaciens （N22) were taken as test materials,bacterial diversity on the surface of grape leaves was studied by PCR-DGGE to clarify the effect of two kinds of bacteria on bacterial diversity.The results showed that the size of approximately 250 bp bacterial 16S rDNA V3 variable region was amplified by PCR with total DNA extracted from the water washed grape leaves as template,17 bands were labeled by DGGE electrophoresis,those were found that 15 known bacteria on the leaves of grape,including Bacillus,Micrococcus,Pseudomonas,Streptomyces,and two uncultured bacteria.The kinds of bacteria after spraying Y24 agent were more abundant,of which four special bands represented Microbacterium,Actinomyces,Pseudomonas flava and Bacillus simplex.Cluster analysis showed that the bacterial diversity on leaves treated with Y24 was significantly different from that of the control and N22 agents,but there was no difference between the treatment of N22 and the control.Phylogenetic analysis showed that the bacterial diversity of grape leaves remained after spraying with bacterial agents.It was conclused that spraying Y24 could significantly improve the bacterial diversity of grape leaves,while N22 had no significant effect on bacterial diversity of grape leaves.

References:

[1]SUSAN S H,CHRISTEN D U.Bacteria in the leaf ecosystem with emphasis on Pseudomonas syringae-a pathogen,ice nucleus,and epiphyte[J].Microbiology and Molecular Biology Reviews，2000,64（3):624-653.

[2]MARTINS G,LAUGA B,MIOT-SERTIER C,et al.Characterization of epiphytic bacterial communities from grapes,leaves,bark and soil of grapevine plants grown,and their relations[J].PLoＳ One,2013,8（8):e73013.
[3]张世伟,陈曦,钟其顶.不同品种酿酒葡萄表皮微生物群落多样性分析[J].生物技术通报,2017,33（3):128-137．
[4]刘蓓一,丁成龙,乔伟艳,等.利用PCR-DGGE技术研究稻草与黑麦草混合青贮过程中菌群的动态变化[J].江苏农业科学,2017,45（11):123-126.
[5]冉淦侨,张红艳,任平.PCR-DGGE技术监测自然发酵葛根中微生物菌群动态变化[J].生物技术通报,2017,33（9):85-93.
[6]刘沛.应用DGGE技术研究低分子量聚乙烯粉末对土壤细菌遗传多样性的影响[D].成都：四川师范大学,2017.
[7]马俊孝,季明杰,孔健.PCR-DGGE技术在微生物物种多样性研究中的局限性及其解决措施[J].食品科学,2008,29（5):493-497.
[8]LEVEAU J H J,TECH J J.Grapevine microbiomics：Bacterial diversity on grape leaves and berries revealed by high-throughput sequence analysis of 16S rRNA amplicons[C].International Symposium on Biological Control of Postharvest Diseases:Challenges and Opportunities.2010:31-42.
[9]BLOOMFIELD S F,STEWART G S A B,DODD C E R,et al.The viable but non-culturable phenomenon explained?〖KG-*4〗[J].Microbiology,1998,144:1-3.
[10]RAPPE M S,GIOVANNONI S J.The uncultured microbial majority[J].Annual Review of Microbiology,2003,57:369-394.
[11]张秀敏,滕忠才,任凯.“非可培养的”细菌研究进展[J].安徽农业科学,2012,40（10):5811-5812，5819.
[12]STEELE H L,STREIT W R.Metagenomics:Advances in ecology and biotechnology[J].FEMS Microbiology Letters,2005,247（2):105-111.
[13]刘慧杰,杨彩云,田蕴.基于PCR-DGGE技术的红树林区微生物群落结构[J].微生物学报,2010,50（7):923-930.
[14]WEST E R,COTHER E J,STEEL C C,et al.The characterization and diversity of bacterial endophytes of grapevine[J].Canadian Journal of Microbiology,2010,56（3):209-216.

Memo

Memo:
-
Last Update: 2018-02-24