|Table of Contents|

Cloning of MsSAG113 Gene From Medicago sativa L. and  Transformation of Arabidopsis thaliana

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年06
Page:
10-15
Research Field:
Publishing date:

Info

Title:
Cloning of MsSAG113 Gene From Medicago sativa L. and  Transformation of Arabidopsis thaliana
Author(s):
XU QiangZENG HuimingXU LixinCHAO Yuehui
(College of Forestry,Beijing Forestry University,Beijing 100083)
Keywords:
Medicago sativaSAG113 geneArabidopsis thalianatransformation
PACS:
-
DOI:
10.11937/bfyy.20172691
Abstract:
Alfalfa was used as material to clone a homologous gene named SAG113 in Arabidopsis thaliana by RT-PCR technique.The MsSAG113 gene was inserted into plasmid 3302Y with seamless ligase constructing a plant expressing vector,and transgenic Arabidopsis thaliana plants with glufosinate-ammonium resistance were screened by floral dip method.The transgenic materials provide the foundation for further study of MsSAG113 gene.The results showed that its coding domain sequence was 849 bp in length encoding 283 amino acids,and PCR and RT-PCR analysis confirmed the transgenic Arabidopsis thaliana plants with MsSAG113 gene were successfully obtained,which preliminary proved transcription and expression of exogenous gene SAG113 in transgenic Arabidopsis thaliana was able.

References:

 

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Last Update: 2018-03-30