|Table of Contents|

Purification Process and Antioxidant Activities of Total Flavonoids From  Bark of Salix discolor

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年07
Page:
105-112
Research Field:
Publishing date:

Info

Title:
Purification Process and Antioxidant Activities of Total Flavonoids From  Bark of Salix discolor
Author(s):
ZHANG Shanshan1; 2; HAN Liwen1; 2; HE Qiuxia1; 2; LI Xiaobin1; 2; ZHANG Xuanming1; 2; LIU Kechun1; 2
(1.Biology Institute, Qilu University of Technology (Shandong Academy of Sciences),Jinan,Shandong 250013；2.Shandong Provincial Engineering Laboratory for Biological Testing Technology, Qilu University of Technology (Shandong Academy of Sciences),Jinan,Shandong 250013)
Keywords:
Salix discolor; total flavonoids; macroporous resins; antioxidant activities
PACS:
-
DOI:
10.11937/bfyy.20172690
Abstract:
Barks of Salix discolor were used as test materials,the content of total flavonoids was determined by UV.Absorption rate and desorption rate were used as indexes,nine kinds of macroporous resins were examined and the best macroporous resin was screened,and optimal purification process of total flavonoids from bark of Salix discolor was determined by optimizing purification conditions and antioxidant activity differences between total flavonoids before and after purification were discussed.The results showed that D101 resin possessed the optimal absorption capacity and desorption capacity.The optimum purification process was as follows,60% ethanol was used for extracting for two times and the concentration of total flavonoids of 1.0 mg?mL-1 was obtained as loading solution,the diameter height ratio of column was 1∶15,the loading speed of solution was 1.0 BV?h-1 with the loading volume of 5 BV and 60% ethanol was used as elution solvent with elution speed of 1.0 BV?h-1,then elution in the eluting scope of 0.8-2.2 BV was collected and the content of total flavonoids after purification was 64.94% and the yield of total flavonoids was 98.34%.DPPH free radical-scavenging activity and ferric-reducing antioxidant power of total flavonoids after purification were near third and twice as much as that of before purification,respectively.D101 macroporous resin showed the best enrichment and purification efficiency for total flavonoids from bark of Salix discolor and antioxidant activities of total flavonoids after purification were higher.This experiment could provide theoretical basis for further development and the improvement of industrial additional value of Salix discolor.

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Last Update: 2018-04-20