|Table of Contents|

Effects of Different Sterilization Methods on Enzyme Activity in  Culture Materials of Hypsizygus marmoreus

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年08
Page:
155-160
Research Field:
Publishing date:

Info

Title:
Effects of Different Sterilization Methods on Enzyme Activity in  Culture Materials of Hypsizygus marmoreus
Author(s):
YANG Hongpeng1; BAN Litong1; SUN Ning1; HUANG Liang1; WANG Yu1; WANG Xufeng2; 3
(1.College of Agronomy ＆ Resources and Environment,Tianjin Agricultural College,Tianjin 300384;2.Tianjin Hongbin hesheng Agricultural Technology Development Limited Company,Tianjin 300400;3.Tianjin Lyushengpengyuan of Agricultural Science and Technology Development Limited Company,Tianjin 300400)
Keywords:
Hypsizygus marmoreus; culture materials; sterilization methods; enzyme activities
PACS:
-
DOI:
10.11937/bfyy.20172663
Abstract:
The culture materials of Hypsizygus marmoreus under the step high pressure sterilization,the ordinary high pressure sterilization and the atmospheric sterilization was used as the test materials.The effect of different sterilization methods on the ability of enzyme from mycelium of Hypsizygus marmoreus was analyzed by UV spectrophotometry and cultivation test.The results showed that the activities of 3 kinds of lignin enzyme and 4 kinds of cellulase from the mycelium of the step sterilized culture medium were the highest.The enzyme activity of mycelia in the step high sterilization culture medium was laccase (315.12 U?L-1),manganese peroxidase (123.27 U?L-1),lignin peroxidase (19.15 U?L-1),filter paper enzyme(132.57 U?L-1),endonuclease(64.02 U?L-1),β-glucosidase (34.37 U?L-1) and hemicellulose (29.02 U?L-1).The agronomic characteristics of Hypsizygus marmoreus from the mycelium of the step high pressure sterilization were higher than that of the ordinary high pressure sterilization and the atmospheric sterilization.The average cluster weight (184.58 g) of the fruiting body from step high pressure sterilization was higher than the average cluster weight of the fruiting body from ordinary high pressure sterilization (174.72 g).The average cluster weight of the fruiting body from atmospheric sterilization (170.24 g) was the lowest.

References:

 

[1]孙培龙,魏红福,杨开,等.真姬菇研究进展[J].食品科技,2009(9):54-56.

[2]上官周建,林汝楷，张运茂.真姬菇生物学特性的研究[J].食用菌学报,2004,11(4):1-7.

[3]杨红澎,班立桐,黄亮,等.阶梯式高压灭菌与常规灭菌方法对蘑菇培养料微量营养素的影响[J].北方园艺,2016(10):141-143.

[4]高玉千,高方,张世敏,等.黑木耳漆酶高产菌株的筛选[J].中国农学通报,2009,25(21):301-304.

[5]池玉杰,闫洪波.红平菇木质素降解酶系统漆酶、锰过氧化物酶及木质素过氧化物酶的检测[J].林业科学,2009,45(12):114-118

[6]GLENN J K,GOLD M H.Purification and characterization of an extracellular Mn (Ⅱ) dependent peroxidase from the lignin-degrading basidiomycete Phanerochaete chrysosporium[J].Archives of Biochemistry and Biophysics,1985,11(242):329-341.

[7]MICHAEL H G,MARGARET A.Molecular biology of lignin-degradation basidiomycete Phanerchaete ysosponium[J].Microbiological Reviews,1993,57(3):605-622.

[8]孙江慧.几株食用菌对秸秆木质纤维素降解能力的研究[D].南京:南京农业大学,2012.

[9]张鹏,王延锋,潘春磊,等.食用菌漆酶生物学性质及其应用研究进展[J].生物技术通报,2014(9):39-44.

[10]邹敏辰,李江华.里氏木霉液体发酵纤维素酶的研究[J].发酵科技通讯,1998,27(1):1-5.

[11]陈文祥.产纤维素酶放线菌的筛选及其产酶条件与酶学性质初探[D].成都:四川师范大学,2007.

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Last Update: 2018-05-10