|Table of Contents|

Determination of Abscisic Acid in Crabapple by High Performance Liquid Chromatography

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年04
Page:
138-144
Research Field:
Publishing date:

Info

Title:
Determination of Abscisic Acid in Crabapple by High Performance Liquid Chromatography
Author(s):
LI Xiaoying; ZHAO Qingqing; LIU Yue; LIU Zhenlin; YANG Junming; ZHANG Guojun
College of Horticulture Science ＆ Technology,Hebei Normal University of Science ＆ Technology,Qinhuangdao,Hebei 066600
Keywords:
crabapple （Malus sp.); high performance liquid chromatography （HPLC); abscisic acid （ABA); content
PACS:
-
DOI:
10.11937/bfyy.20172564
Abstract:
‘Strawberry jelly’ crabapple （Malus sp.) was used as test material,a reliable method for determination of abscisic acid （ABA) content in crabapple was established using the high performance liquid chromatography （HPLC) combined with solid phase extraction（SPE).By optimizing the sample pre-treatment and the chromatographic conditions,the final experimental process as follows,sample weight for 3 g,with 80% precooling methanol extracting for 12 hours,using the insoluble PVPP for adsorbing the phenolic compounds,then the extracts was decolorize by petroleum ether,and ABA was extracted by ethyl acetate,then further purification using C18 cartridge.The method was performed with a reverse-phase C18 column and using the mixture of methanol and water （containing 1.0% acetic acid) with the volume ratio of 45∶55 as mobile phase；the sample injection volume was 20 μL and the flow rate was 1.0 mL?min-1；the detection wavelength was 254 nm and the column temperature was 45 ℃.The results showed that the ABA were separated well by this method,which calibration curves had good linearity over the range from 0.01 to 0.50 μg?mL-1 with a correlation coefficient of 0.999 8,the average recovery rate for ABA was larger than 70% at three concentration levels.The abscisic acid content was lowest as 0.026 9 μg?g-1,and the highest as 0.357 9 μg?g-1 with great difference,at the concentration level of previous reported.This method showed great significance for the further research of crabapples breeding and growth.

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Last Update: 2018-02-27