|Table of Contents|

Calli Induction Techniques of Danshui Pear Leaf in vitro(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年22
Page:
62-66
Research Field:
Publishing date:

Info

Title:
Calli Induction Techniques of Danshui Pear Leaf in vitro
Author(s):
ZENG Lingda12HUANG Yumin1XU Chunxiang3LI Caihua1
1.Department of Life Science,Huizhou University,Huizhou,Guangdong 516007;2.Institute of Biotechnology,Huizhou University,Huizhou,Guangdong 516007;3.College of Horticulture,South China Agriculture University,Guangzhou,Guangdong 510642
Keywords:
Danshui pearleaf in vitrocallusculture medium
PACS:
-
DOI:
10.11937/bfyy.20172255
Abstract:
Leaf of Danshui pear was used as explant,MS and White medium as the basic medium,the plant growth regulator 6-BA and NAA and different inoculation methods were studied to obtain callus of Danshui pear.The results showed that leaf callus induction rate of the Danshui pear in MS and White culture medium were 72.4% and 30.9%,the best callus induction formula was MS+6-BA1.0 mg?L-1+NAA 0.2 mg?L-1,20 days leaves the highest callus induction rate was 100.0%,40 days leaves the highest callus induction rate was 60.0%,abaxial surface of flat leaf callus induction rate was 100.0%,the adaxial flat rate of callus induction was 78.3%,when the line of leaf callus induction rate was 50.0%.These results indicate that MS medium was better than White medium for leaf calli induction,younger leaves had higher callus initiation rates,leaf abaxial side in contact with the medium was more suitable for inducing leaf callus in vitro.

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Last Update: 2017-11-22