Induction and Anti-browning of Leaf Callus From Mentha piperita L.
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2018年17
- Page:
- 145-150
- Research Field:
- Publishing date:
Info
- Title:
- Induction and Anti-browning of Leaf Callus From Mentha piperita L.
- Author(s):
- HU Yanmei1; JIANG Guangyu2; FANG Zhongming2; 3
- (1.Journal Department,Jianghan University,Wuhan,Hubei 430056；2.Life Science and Technology College，Wuhan Bioengineering Institute,Wuhan,Hubei 430415;3.Center of Applied Biotechnology,Wuhan Bioengineering Institute,Wuhan,Hubei 430415)
- Keywords:
- Mentha piperita L.; tissue culture; callus; browning; kinetin（KT）; activated carbon（AC）
- PACS:
- -
- DOI:
- 10.11937/bfyy.20172146
- Abstract:
- Taking Mentha piperita L.as test material,the effect of callus induction on the leaves of Mentha piperita L.plantlets was studied to provide the basis for establishment of suspension system and regeneration system.The basice mediums,the plant regulator combination with 6-BA,KT and 2,4-D,NAA and antibrowning agents were detected for the callus induction and formation.The results showed that B5 as the basic medium and plant growth regulator KT 1.0 mg·L-1+2,4-D 0.5 mg·L-1 combination were suitable for callus induction and formation；the planet leaves callus induction rate was 100%,browning rate was 0.0%,the callus biomass reached 42.1 g·L-1 on the medium of B5+KT 1.0 mg·L-1+2,4-D 0.5 mg·L-1 with AC 0.2 g·L-1.The callus system was established by this experiment.
Last Update: 2018-10-16