|Table of Contents|

Analysis of Different Plant Hormone on Callus Induction Quality of Sophora davidii Leaf

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年10
Page:
63-69
Research Field:
Publishing date:

Info

Title:
Analysis of Different Plant Hormone on Callus Induction Quality of Sophora davidii Leaf
Author(s):
WU Lifang1; 2; WEI Xiaomei1; 2; YING Qianqian1; YANG Cuirong1; YE Men1
(1.College of Biological Resource and Food Engineering,Qujing Normal University,Qujing,Yunnan 655011；2.Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution For Animals and Plants on Yun-Gui Plateau,Qujing,Yunnan 655011)
Keywords:
Sophora davidii; growth regulator; callus; leaf; orthogonal design
PACS:
-
DOI:
10.11937/bfyy.20172145
Abstract:
To establish excellent callus induction culture system,the no vaccine leaves of Sophora davidii were used as materials to research effect of different growth regulators on callus induction by double factors orthogonal design of experiment.The aim was to screen the best callus induced medium in order to provide a theoretical basis for tissue culture of Sophora davidii. The results showed that 2,4-D and 6-BA combination could induce relatively loose callus on the MS culture medium.Callus surface had protrusions and green bud points on the part of the callus.2,4-D combined with KT could also induce the formation of callus,but the growth and loose degree of callus was less than 2,4-D and 6-BA combination.NAA and 6-BA combination,NAA and KT combination could induce callus formation early in the leaf edge,callus brown gradually after culture 45 days.MB and B5 could induce callus,callus color was slightly green on the basic medium.2,4-D was advantageous to callus induction of Sophora davidii. The best callus induction medium was MS+2,4-D 1.0 mg?L-1+6-BA 1.0 mg?L-1+4% sucrose+0.7% agar,the induction rate was 75.58%,callus grew earlier,osteoporosis,vigorous growth in the whole experiment.

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Last Update: 2018-06-11