|Table of Contents|

Optimization of Extraction Process and in vitro Antioxidant Activities of Flavonoid From Selaginella tamariscina(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年21
Page:
134-139
Research Field:
Publishing date:

Info

Title:
Optimization of Extraction Process and in vitro Antioxidant Activities of Flavonoid From Selaginella tamariscina
Author(s):
ZHANG Hao1; 2; YE Jia1; 2; HAO Lihua3; ZHENG Yunpu3; DONG Tianyu1; WU Zilong1; 2
1.School of Life Science and Engineering,Handan University,Handan,Hebei 056005;2.Research Center for Plant Resources Applied Technology in Jinan Mountain at University of Hebei Province,Handan,Hebei 056005;3.School of Water Conservancy and Hydropower,Hebei University of Engineering,Handan,Hebei 056038
Keywords:
Selaginella tamariscina Spring; flavonoids; antioxidant activity; orthogonal array design; superoxide anion
PACS:
-
DOI:
10.11937/bfyy.20171938
Abstract:
In order to investigate the optimal process parameters for extracting flavonoids from Selaginella tamariscina and its antioxidant activities,single factor experiments and an orthogonal array design L9(34) were performed in combination to optimize process parameters for the extraction of flavonoids from Selaginella tamariscina.Meanwhile,the scavenging effects of flavonoids on the superoxide anion were also examined in the current study.The results showed that the extraction yield of flavonoids was 15.7% with the optimal parameters that the ethanol concentration,solid-liquid ratio,extraction time,and extraction temperature were 60%,1∶15 g?mL-1,30 minutes,and 70 ℃,respectively.Moreover,it was found that the extraction from Selaginella tamariscina characterized by high antioxidant ability for scavenging the superoxide anion with the maximum scavenging ratio at the extraction concentration of 3.0 mg?mL-1.These results not only provided data for further optimizing the extraction parameters of flavonoids from Selaginella tamariscina,but also had important significance for understanding the antioxidant mechanisms and high efficiency utilization of plant resources.

References:

[1]延玺,刘会青,邹永青,等.黄酮类化合物生理活性及合成研究进展[J].有机化学,2008(9):1534-1544.

[2]许晖,孙兰萍,张斌,等.花生壳黄酮类化合物的研究进展[J].农产品加工,2008(3):11-15.
[3]董晓宁,赵海福,赵强,等.中草药提取物抗氧化作用及其机理研究进展[J].中兽医医药杂志,2014(1):25-29.
[4]陈梅霞,苏洁,张玥莉,等.银杏叶提取物与银杏黄酮对高脂血症大鼠血脂水平影响的比较研究[J].中国新药杂志,2014(7):833-838.
[5]李洪玉,孙静芸.竹叶化学成分研究[J].中药材,2003,26(8):562-563.
[6]罗金岳,陈小燕.从箬竹叶中提取茶多酚的研究[J].林产化工通讯,2003,37(6):15-19.
[7]张英,吴晓琴,俞卓裕.竹叶黄酮和内酯的季节性变化规律研究[J].林业化学与工业,2002(22):65-69.
[8]周荣汉.药用植物化学分类学[M].上海:科学技术出版社,1988:123-141.
[9]黄文,王益.竹叶提取物抑菌特性的研究[J].林产化学与工业,2002(22):68-70.
[10]庞战军,周玖,陈瑗.自由基医学研究方法[M].北京：人民卫生出版社,2000：7-217.
[11]CAYUELA M M.Oxygen free radicals and human disease[J].Biochimie,1995,77:147-161.
[12]MARNETT L J.Oxygen radicals,lipid peroxidation and DNA damage[J].Toxicology,2002,181:219-222.
[13]中国植物志编辑委员会.中国植物志(第一卷)[M].北京:科学出版社,1959.
[14]牛玉璐,赵建成.河北茅荆坝自然保护区蕨类植物资源的调查研究[J].衡水学院学报,2007(1):1-4.
[15]毕跃峰,郑晓珂,史社坡,等.卷柏属植物化学成分与药理活性[J].国外医药(植物药分册),2002（3):97-100.
[16]黎莉,陈科力,朱田密,等.卷柏属7种药用植物的提取物抑制黄嘌呤氧化酶的活性研究[J].中药材,2007（4):445-447.
[17]张红伟,孙晓飞,田景奎.卷柏属植物黄酮类成分研究概况[J].亚太传统医药,2007(10):63-65.
[18]张红梅,景颖,张国刚,等.卷柏中双黄酮类化学成分及卷柏属该类化合物的核磁共振特征[J].中南药学,2011（6):419-423.
[19]SLOLEY B D,URICHUK L J,MORLEY P,et al.Identification of kaempferol as a monoamine oxidase inhibitor and potential neuroprotectant in extracts of Ginkgo biloba leaves[J].Pharm Pharmacol,2000,52:451.
[20]CHEN X Y,LIU P.Mocular biology and genetic engineering of plant secondary metabolism[J].Life Science,1996（2):8211.
[21]哈森其木格.分光光度法测定蒙药卷柏中总黄酮含量[J].光谱实验室,2005(2):445-448.
[22]郑晓珂,侯庆伟,李民,等.卷柏总黄酮提取工艺研究[J].中国新药杂志,2011(16):1509-1513.
[23]赵昕,吴子龙,张浩,等.减压法提取中华卷柏总黄酮的研究[J].北方园艺,2011(15):217-219.
[24]林丹英,尤婷婷,黄锁义.茼蒿总黄酮提取及对羟自由基清除作用[J].中国野生植物资源,2007(26):57-59.

Memo

Memo:
-
Last Update: 2017-11-14