|Table of Contents|

Isolation,Identification and Antibiotic Activities of Endophytes in Gentiana stipitata

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年01
Page:
135-139
Research Field:
Publishing date:

Info

Title:
Isolation,Identification and Antibiotic Activities of Endophytes in Gentiana stipitata
Author(s):
JIN Yu1; REN Mengnan1; YANG Hongpeng1; WEI Zhuo1; QUE Sheng2; HUANG Haidong1
1.College of Agronomy & Resources and Environment,Tianjin Agricultural University,Tianjin 300384;2.Normal College for Nationalities,Qinghai Normal University,Xining,Qinghai 810008
Keywords:
endophyte; Gentiana stipitata; Paenibacillus; antibiotic activities
PACS:
-
DOI:
10.11937/bfyy.20171784
Abstract:
Taking Gentiana stipitata as material.An endophytic bacterium with antibacterial activity was isolated by the method of tissue block separation and gradient dilution.Active substance of endophyte and relevance between its and active stubstance of Gentiang stipitata were analyzed,to provided foundation for the development and utilization of endophytes resources from medicinal plants.The results showed that endophytes WZ-6 had significant inhibitory effect on Staphylococcus aureus and Corynespora cassiicola.According to the characteristics of morphology and 16S rDNA phylogenetic analysis,strain WZ-6 was identified as species of genus Paenibacillus,and showed the highest similarity to Paenibacillus terrae,reaching 98.9%.The results of HPLC analysis showed that the fermented product of strain WZ-6 contained antimicrobial active component of iridoid.

References:

[1]HIRONOBU M,HISAO M.Endophytic bacteria in the rice plant[J].Microbes and Environments,2008,23（2):109-117.

[2]李俊峰,刘文洪,张贝贝,等.铁皮石斛内生菌的分离及代谢产物活性的初步研究[J].中华中医药杂志,2016,3（31):970-974.
[3]丁锐,李萌凯,任光宇,等.羊耳蒜内生真菌的分离鉴定与抑菌活性筛选[J].北方园艺,2017（1):126-130.
[4]刘海燕,杜丰玉,肖琳.萝芙木内生真菌的分离及发酵产物抑菌活性的研究[J].北方园艺,2016（15):105-107.
[5]ZHAO Z L,GAAWE D,WANG Z T.Identification of medicinal plants used as Tibetan Traditional Medicine Jie-Ji[J].Journal of Ethnopharmacology,2010,132（1):122-126.
[6]马羚.藏药“榜间嘎保”品种整理及主流品种的品质研究[D].成都:成都中医药大学,2014.
[7]CHUN Y X,DE Z L.Use of DNA barcode sensu lato to identify traditional Tibetan medicinal plant Gentianopsis paludosa（Gentianaceae)[J].Systematics and Evolution,2011,49（3):267-270.
[8]董晓蕾,张霁,赵艳丽,等.龙胆炮制前后化学成分变化的比较研究[J].药物分析杂志,2015,35（4):620-626.
[9]李姗,赵野,刘圆,等.高速逆流色谱法一次性分离制备藏药蓝玉簪龙胆中的三个化合物[J].西南民族大学学报（自然科学版),2016,42（6):660-664.
[10]JUNG H Y,HEE M O,BYUNG D Y,et al.Paenibacillus kribbensis sp.nov.and Paenibacillus terrae sp.nov.,bioflocculants for efficient harvesting of algal cells[J].Systematic and Evolutionary Microbiology,2003,53:295-301.
[11]夏天.Paenibacillu ssp.Aloe-11全基因组测序及其比较基因组学研究[D].重庆:西南大学,2012.
[12]杨红澎,确生,吴锡冬,等.蓝玉簪龙胆中苷类成分的研究[J].中国中药杂志,2008,33（21):2505-2507.
[13]孙宇佳,唐淑含,王姗姗.环烯醚萜类化合物的结构分类及生物活性[J].黑龙江科技信息,2014,1（2):80.
[14]郎楷永.中国的龙胆[J].科技导报,2008（1):64.
[15]张丽红,刘艳芳,胡青平.中国南海一株固氮类芽孢杆菌的筛选和分离鉴定[J].海洋科学,2015,39（3):100-105.
[16]鲁红学,周炎.类芽孢杆菌在植物病害防治和环境治理中的应用研究进展[J].安徽农业科学,2008,36（30):13244-13247.

Memo

Memo:
-
Last Update: 2018-01-12