|Table of Contents|

Cloning and Bioinformatic Analysis of DFR Gene From Iris lactea Pall.var.chinensis (Fisch.) Koidz(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年24
Page:
109-115
Research Field:
Publishing date:

Info

Title:
Cloning and Bioinformatic Analysis of DFR Gene From Iris lactea Pall.var.chinensis (Fisch.) Koidz
Author(s):
HU Juan1; 2; AN Shaoya1; 2; LIN Zhe1; 2; ZHANG Hong1; 2; BAO Han1; 2; CHEN Ren1; 2
1.Key Lab of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China,Ningxia University,Yinchuan,Ningxia 750021;2.School of Life Science,Ningxia University,Yinchuan,Ningxia 750021
Keywords:
Iris lactea Pall.var.chinensis; transcriptome sequencing; anthocyanin biosynthesis; dihydvroflavonol-4-reductase; bioinformatic analysis
PACS:
-
DOI:
10.11937/bfyy.20171745
Abstract:
Iris lactea Pall.var.chinensis (Fisch.) Koidz petals were used as material for transcriptome sequencing.According to the annotation data,a key gene encodes dihydvroflavonol-4-reductase (DFR) in its anthocyanin biosynthesis was cloned by using PCR amplification and was charactered using bioinformatic analysis.The full-length of DFR gene of I.lactea var.chinensis consisted of 1 427 bp encoding a protein with 357 amino acid residues (designated as IlDFR,accession No.KY907171).Based on the comparison of the deduced amino acid sequence,IlDFR shared high identity with other plant DFRs,and had high homology with congeneric Iris hollandica DFR.IlDFR had a predicted molecular mass of 39.99 kDa and an isoelectric point of 5.89.It mainly composed of α-helixes and β-sheets,and was considered as an acidic,hydrophilic,no signal peptide and labile protein that located in the cytoplasm.IlDFR had a ‘VTGASGYVGSWLVMKLLRDGY’ amino acid sequence that was similar to the NADPH binding domain which was a typical conserved sequence in most of other plant DFRs,but differenced in 4 amino acid residues.

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Last Update: 2017-12-29