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Molecular Identification of Powdery Mildew of  Medicinal Herb Galium aparine(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2018年09
Page:
149-152
Research Field:
Publishing date:

Info

Title:
Molecular Identification of Powdery Mildew of  Medicinal Herb Galium aparine
Author(s):
PEI Dongli1; ZHU Xiaoqin1; WANG Shunan1; WANG Yujing1; LI Chengwei2
(1.School of Biology and Food Sciences，Shangqiu Normal University/Key Laboratory of Plant-Microbe Interactions，Shangqiu，Henan 476000；2.School of Life Science and Technology，Henan Institute of Science and Technology，Xinxiang，Henan 453003)
Keywords:
Galium aparine; powdery mildew;  ITS sequences;  morphological identification; etiological identification; molecular systematics
PACS:
-
DOI:
10.11937/bfyy.20171623
Abstract:
Galium aparine powdery mildew detected in Shangqiu of Henan was used as experiment material，morphological，etiological and molecular systematic identification，and phylogenetic evolution relatiognship of the powdery mildew were identified by molecular systematics methods，in order to provide a theoretical basis for its prevention and treatment.The results showed that the powdery mildew was grown on two sides of leaves and stems，then covering the whole leaf surface with the pathogen progress.The conidiophores were erected，(100-260)μm×(10-12)μm，with 2-3 immature spores.The foot cells were (28-65)μm×(9-11)μm，next to 2-3 short cells.The spores were cylindrical，(22-42)μm×(15-20)μm.Internal transcribed spacer (ITS) sequence of the pathogen ribosomal DNA was amplified by PCR and sequenced to obtain the 632 bp with the GenBank accession number KX396590 and sequence analysis by MEGA 5.0 software revealed this ITS sequence and one sequence of Golovinomyces orontii (GenBank accession number AB430813) were gathered together，distantly related to the ITS sequence of other five species in the same genus.The results showed that pathogen of G.aparine powdery mildew in Shangqiu was G.orontii.

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Last Update: 2018-05-25