|Table of Contents|

Classification and Identifition of Mycorrhizal Fungi of
Paphiopedilum micranthum
(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年24
Page:
116-122
Research Field:
Publishing date:

Info

Title:
Classification and Identifition of Mycorrhizal Fungi of
Paphiopedilum micranthum
Author(s):
TIAN Fan1; LIAO Xiaofeng2; WANG Lianhui1; ZHU Guosheng3; GUI Yang3; BAI Xinxiang4
1.Guizhou Forestry Academy,Guiyang,Guizhou 550005;2.Institute of Mountain Resources,Guizhou Academy of Sciences,Guiyang，Guizhou 550001;3.Institute of Morden Chinese Medical Materials,Guizhou Academy of Agricaltural Sciences,Guiyang,Guizhou 550006;4.College of Forestry,Guizhou University，Guiyang,Guizhou 550025
Keywords:
Paphiopedilum micranthum; mycorrhizal fungi; Rhizoctonia; rDNA ITS analysis; identifition
PACS:
-
DOI:
10.11937/bfyy.20171417
Abstract:
Classification and identification of mycorrhizal were associated with Paphiopedilum micranthum,the method of morphological was combined with the ITS gene fragments were PCR amplified and sequenced,the rDNA ITS regions were analyzed by means of the software of NCBI BLAST.The results showed that the 29 strains with different micro- and macro-morphological.Secondly,rDNA-ITS sequence analysis of 29 predo minant strains.The fungi were identified into 23 genus and 1 family,23 strains belong to Epulorhiza,Tulasnella,Rhizoctonia,Fusarium,Trametes,Trichaptum,Daedaleopsis,Psathyrella,Fomitopsi,Chaetomium,Pleurotus,Lecanicillium,Eutypella,Lecanicillium,Peniophorella,which their frequency were 16% and 19%.Only one family belonged to Tulasnellaceae.12 strains of dominant mycorrhiza had different microcosmic and macroscopic,none of them had conidiophore,near the hyphal branching-points formed of septum and conjugate nuclei,11 strains mycorrhiza with intestiniform cells,the most of mycorrhiza had a distinct aroma.

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Last Update: 2017-12-29