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Cloning and Sequence Analysis of Coat Protein Genes of CMV,TMV and PVS From Lycopersicon esculentum Mill.Plant(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年22
Page:
12-18
Research Field:
Publishing date:

Info

Title:
Cloning and Sequence Analysis of Coat Protein Genes of CMV,TMV and PVS From Lycopersicon esculentum Mill.Plant
Author(s):
YU Peixia1; ZHAO Huiqi1; LIU Yong2; WANG Defu1; NIU Yanbing1
1.College of Life Sciences,Shanxi Agricultural University,Taigu,Shanxi 030801；2.Hunan Plant Protection Institute,Hunan Academy of Agricultural Science,Changsha,Hunan 410125
Keywords:
Lycopersicon esculentum Mill.; mixed infection; tobacco mosaic virus; cucumber mosaic virus; potato virus S
PACS:
-
DOI:
10.11937/bfyy.20171234
Abstract:
The leaves of tomato (Lycopersicon esculentum) from Jinzhong region of Shanxi Province shown chlorotic yellows,curly,black leaf pathogen were used as materials,the technology of double-stranded RNA (dsRNA) and sequence-independent amplification(SIA) was used to identify the virus pathogen that induced tomato,in order to provide reference for the prevention and control of virus disease infecting tomato in Shanxi Province.The results showed that samples of plants were infected with tobacco mosaic virus(TMV),cucumber mosaic virus(CMV) and potato virus S(PVS) respectively and named CMV-SXFQ,TMV-FQ and PVS-SXFQ.To further characterize the isolate of CMV-SXFQ,TMV-FQ and PVS-SXFQ,the coat protain(CP) of CMV-SXFQ,TMV-FQ and PVS-SXFQ were cloned and analyzed.Sequence alignments showed that the nucleotide homology similarity between CMV-SXFQ and CMV subgroup virus were 74.9%-98.8%,and the amino acide sequence similarity were 82.5%-99.5%.The nucleotide homology similartity between TMV-FQ and TMV subgroup virus was 74.4%-99.5%,and the amino acide sequence similarity were 83.6%-100.0%.The nucleotide homology similartity between PVS-SXFQ and PVS subgroup virus were 93.3%-99.3%,and the amino acide sequence similartity were 92.4%-100.0%.Phylogenetic analysis suggested that CMV-SXFQ was the same as CH,HLJ,XJ2,Am and As,which belonged to the CMV subgroup I B;TMV-FQ was closely related to IM,Jimo and SXFQ;PVS-SXFQ was closely related to Cm,St.

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Last Update: 2017-11-22